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Monosialoganglioside 1 may alleviate neurotoxicity induced by propofol combined with remifentanil in neural stem cells
- Source :
- Neural Regeneration Research, Neural Regeneration Research, Vol 12, Iss 6, Pp 945-952 (2017)
- Publication Year :
- 2017
- Publisher :
- Medknow Publications & Media Pvt Ltd, 2017.
-
Abstract
- Monosialoganglioside 1 (GM1) is the main ganglioside subtype and has neuroprotective properties in the central nervous system. In this study, we aimed to determine whether GM1 alleviates neurotoxicity induced by moderate and high concentrations of propofol combined with remifentanil in the immature central nervous system. Hippocampal neural stem cells were isolated from newborn Sprague-Dawley rats and treated with remifentanil (5, 10, 20 ng/mL) and propofol (1.0, 2.5, 5.0 μg/mL), and/or GM1 (12.5, 25, 50 μg/mL). GM1 reversed combined propofol and remifentanil-induced decreases in the percentage of 5-bromodeoxyuridine(+) cells and also reversed the increase in apoptotic cell percentage during neural stem cell proliferation and differentiation. However, GM1 with combined propofol and remifentanil did not affect β-tubulin(+) or glial fibrillary acidic protein(+) cell percentage during neural stem cell differentiation. In conclusion, we show that GM1 alleviates the damaging effects of propofol combined with remifentanil at moderate and high exposure concentrations in neural stem cells in vitro, and exerts protective effects on the immature central nervous system.
- Subjects :
- 0301 basic medicine
proliferation
Central nervous system
Remifentanil
nerve regeneration
monosialoganglioside 1
propofol
remifentanil
neural stem cells
neurotoxicity
neuroprotection
differentiation
apoptosis
[Ca2+]i
neural regeneration
Pharmacology
Neuroprotection
lcsh:RC346-429
03 medical and health sciences
Developmental Neuroscience
medicine
lcsh:Neurology. Diseases of the nervous system
Glial fibrillary acidic protein
biology
Chemistry
Neurotoxicity
medicine.disease
Neural stem cell
030104 developmental biology
medicine.anatomical_structure
Apoptosis
Anesthesia
biology.protein
Propofol
medicine.drug
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 18767958 and 16735374
- Volume :
- 12
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Neural Regeneration Research
- Accession number :
- edsair.doi.dedup.....f18f7e3b906f5e842a9d99ce6907d407