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Long Intergenic Noncoding RNA HOTAIR Is Overexpressed and Regulates PTEN Methylation in Laryngeal Squamous Cell Carcinoma
- Source :
- The American Journal of Pathology. 182:64-70
- Publication Year :
- 2013
- Publisher :
- Elsevier BV, 2013.
-
Abstract
- Homeobox (HOX) transcript antisense RNA (HOTAIR) is a long intergenic noncoding RNA (lincRNA) that is significantly overexpressed in breast and hepatocellular cancers. It remains unclear, however, whether HOTAIR plays an oncogenic role in human laryngeal squamous cell cancer (LSCC). We therefore investigated the expression and functional role of HOTAIR in LSCC. HOTAIR levels were significantly higher in LSCC than in corresponding adjacent non-neoplastic tissues, and patients with poor histological grade or advanced clinical stage had higher HOTAIR expression. Log-rank test showed a significant association between high levels of HOTAIR and poor prognosis in LSCC patients. Multivariate Cox analysis suggested that HOTAIR is an independent prognostic factor of LSCC. siRNA-mediated knockdown of HOTAIR led to reduced invasion and increased apoptosis of Hep-2 cells in vitro and significantly reduced growth of LSCC xenograft tumors in mice. Moreover, PTEN methylation was significantly reduced in Hep-2 cells depleted of HOTAIR. Taken together, these results suggest that the oncogenic role of HOTAIR in LSCC is related to promotion of PTEN methylation. HOTAIR could serve as a marker for LSCC prognosis and a potential target for therapeutic intervention.
- Subjects :
- Male
Transplantation, Heterologous
Apoptosis
Pathology and Forensic Medicine
Mice
Biomarkers, Tumor
Tumor Cells, Cultured
Animals
Humans
PTEN
Neoplasm Invasiveness
RNA, Neoplasm
Hox gene
Laryngeal Neoplasms
Mice, Inbred BALB C
Gene knockdown
biology
PTEN Phosphohydrolase
HOTAIR
DNA, Neoplasm
Methylation
DNA Methylation
Middle Aged
Prognosis
Non-coding RNA
Gene Expression Regulation, Neoplastic
Transplantation
Gene Knockdown Techniques
Carcinoma, Squamous Cell
biology.protein
Cancer research
Female
RNA, Long Noncoding
Neoplasm Transplantation
HOX Transcript Antisense RNA
Genes, Neoplasm
Subjects
Details
- ISSN :
- 00029440
- Volume :
- 182
- Database :
- OpenAIRE
- Journal :
- The American Journal of Pathology
- Accession number :
- edsair.doi.dedup.....f1a1d36d2ca950c8cb2fe55c469bb709