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Nano-mole scale sequential signal assignment by 1H-detected protein solid-state NMR
- Source :
- Chemical Communications. 51:15055-15058
- Publication Year :
- 2015
- Publisher :
- Royal Society of Chemistry (RSC), 2015.
-
Abstract
- We present a 3D (1)H-detected solid-state NMR (SSNMR) approach for main-chain signal assignments of 10-100 nmol of fully protonated proteins using ultra-fast magic-angle spinning (MAS) at ∼80 kHz by a novel spectral-editing method, which permits drastic spectral simplification. The approach offers ∼110 fold time saving over a traditional 3D (13)C-detected SSNMR approach.
- Subjects :
- Chemistry
Proton Magnetic Resonance Spectroscopy
Metals and Alloys
Analytical chemistry
Proteins
Protonation
General Chemistry
Time saving
Signal
Article
Catalysis
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
Solid-state nuclear magnetic resonance
Nano
Mole
Materials Chemistry
Ceramics and Composites
Spinning
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi.dedup.....f1cd95f311e8811b1053ccfd45f24c88