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Curcumin Prevents Aflatoxin B1 Hepatoxicity by Inhibition of Cytochrome P450 Isozymes in Chick Liver
- Source :
- Toxins, Toxins, Vol 8, Iss 11, p 327 (2016), Toxins; Volume 8; Issue 11; Pages: 327
- Publication Year :
- 2016
- Publisher :
- MDPI, 2016.
-
Abstract
- This study was designed to establish if Curcumin (CM) alleviates Aflatoxin B1 (AFB1)-induced hepatotoxic effects and to determine whether alteration of the expression of cytochrome P450 (CYP450) isozymes is involved in the regulation of these effects in chick liver. One-day-old male broilers (n = 120) were divided into four groups and used in a two by two factorial trial in which the main factors included supplementing AFB1 (< 5 vs. 100 μg/kg) and CM (0 vs. 150 mg/kg) in a corn/soybean-based diet. Administration of AFB1 induced liver injury, significantly decreasing albumin and total protein concentrations and increasing alanine aminotransferase and aspartate aminotransferase activities in serum, and induced hepatic histological lesions at week 2. AFB1 also significantly decreased hepatic glutathione peroxidase, catalase, and glutathione levels, while increasing malondialdehyde, 8-hydroxydeoxyguanosine, and exo-AFB1-8,9-epoxide (AFBO)-DNA concentrations. In addition, the mRNA and/or activity of enzymes responsible for the bioactivation of AFB1 into AFBO—including CYP1A1, CYP1A2, CYP2A6, and CYP3A4—were significantly induced in liver microsomes after 2-week exposure to AFB1. These alterations induced by AFB1 were prevented by CM supplementation. Conclusively, dietary CM protected chicks from AFB1-induced liver injury, potentially through the synergistic actions of increased antioxidant capacities and inhibition of the pivotal CYP450 isozyme-mediated activation of AFB1 to toxic AFBO.
- Subjects :
- 0301 basic medicine
Male
Aflatoxin
chicks
Antioxidant
Aflatoxin B1
Curcumin
Health, Toxicology and Mutagenesis
medicine.medical_treatment
lcsh:Medicine
CYP450
Pharmacology
Biology
Toxicology
Article
03 medical and health sciences
chemistry.chemical_compound
DNA Adducts
Cytochrome P-450 Enzyme System
curcumin
aflatoxin B1
AFBO–DNA
medicine
Animals
Cytochrome P-450 Enzyme Inhibitors
RNA, Messenger
CYP2A6
Liver injury
030102 biochemistry & molecular biology
lcsh:R
CYP1A2
Glutathione
Malondialdehyde
medicine.disease
Isoenzymes
030104 developmental biology
chemistry
Biochemistry
Liver
biology.protein
Carcinogens
Microsomes, Liver
Chemical and Drug Induced Liver Injury
Chickens
Peroxidase
Subjects
Details
- Language :
- English
- ISSN :
- 20726651
- Volume :
- 8
- Issue :
- 11
- Database :
- OpenAIRE
- Journal :
- Toxins
- Accession number :
- edsair.doi.dedup.....f20bdad1526715b45dd4d5848e197d85