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Increased glutathione contributes to stress tolerance and global translational changes in Arabidopsis
- Source :
- The Plant Journal. 83:926-939
- Publication Year :
- 2015
- Publisher :
- Wiley, 2015.
-
Abstract
- SUMMARY Although glutathione is well known for its reactive oxygen species (ROS) scavenging function and plays a protective role in biotic stress, its regulatory function in abiotic stress still remains to be elucidated. Our previous study showed that exogenously applied reduced glutathione (GSH) could improve abiotic stress tolerance in Arabidopsis. Here, we report that endogenously increased GSH also conferred tolerance to drought and salt stress in Arabidopsis. Moreover, both exogenous and endogenous GSH delayed senescence and flowering time. Polysomal profiling results showed that global translation was enhanced after GSH treatment and by the induced increase of GSH level by salt stress. By performing transcriptomic analyses of steady-state and polysome-bound mRNAs in GSH-treated plants, we reveal that GSH has a substantial impact on translation. Translational changes induced by GSH treatment target numerous hormones and stress signaling molecules, which might contribute to the enhanced stress tolerance in GSH-treated plants. Our translatome analysis also revealed that abscisic acid (ABA), auxin and jasmonic acid (JA) biosynthesis, as well as signaling genes, were activated during GSH treatment, which has not been reported in previously published transcriptomic data. Together, our data suggest that the increased glutathione level results in stress tolerance and global translational changes.
- Subjects :
- Arabidopsis
Germination
Flowers
Plant Science
Sodium Chloride
Transcriptome
chemistry.chemical_compound
Gene Expression Regulation, Plant
Stress, Physiological
Genetics
Abscisic acid
chemistry.chemical_classification
Reactive oxygen species
biology
Arabidopsis Proteins
Abiotic stress
Jasmonic acid
Cell Biology
Glutathione
Biotic stress
Plants, Genetically Modified
biology.organism_classification
Droughts
Cysteine Endopeptidases
chemistry
Biochemistry
Mutation
Abscisic Acid
Subjects
Details
- ISSN :
- 1365313X and 09607412
- Volume :
- 83
- Database :
- OpenAIRE
- Journal :
- The Plant Journal
- Accession number :
- edsair.doi.dedup.....f24ca968b1b81d2d55dcb4c7047c240f