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Evaluation of Staining Methods for Cytologic Diagnosis of Oral Lesions
- Source :
- Acta Cytologica. 52:697-701
- Publication Year :
- 2008
- Publisher :
- S. Karger AG, 2008.
-
Abstract
- To compare the efficacy ofPapanicolaou, hematoxylin-eosin (H-E), Leishman and periodic acid-Schiff (PAS) staining for cytologic diagnosis of oral lesions.Patients from the Discipline of Stomatology, São José dos Campos Dental School, from the wards of Hosapital Heliópolis and from the dentistry outpatient clinic of the University Hospital, University of São Paulo Medical School, with the following diseases, were selected: erythematous candidiasis (n=9), pseudomembranous candidiasis (n=10), squamous cell carcinoma (n=19), herpes simplex (n=8), paracoccidioidomycosis (n=8) and pemphigus vulgaris (n=1).The different staining methods were compared regarding the quality of definition of cytoplasmic and nuclear morphologic characteristics and the identification of bacteria, fungi, inflammatory cells and secretions. Papanicolaou and H-E staining were considered better methods. In cases of fungal infections, PAS staining is useful and should be applied as a complementary method.Within the limitations of this study, it can be concluded that the cytologic diagnosis of oral lesions along with different staining methods is a useful tool for oral diagnosis.
- Subjects :
- Vaginal Smears
medicine.medical_specialty
Pathology
Histology
Staining and Labeling
business.industry
Cytodiagnosis
Diagnosis, Oral
Candidiasis
Herpes Simplex
General Medicine
Periodic Acid-Schiff Reaction
Dermatology
Pathology and Forensic Medicine
Staining
Cytology
Carcinoma, Squamous Cell
medicine
Eosine Yellowish-(YS)
Humans
Paracoccidioidomycosis
Oral Diagnosis
Hematoxylin
Mouth Diseases
business
Subjects
Details
- ISSN :
- 19382650 and 00015547
- Volume :
- 52
- Database :
- OpenAIRE
- Journal :
- Acta Cytologica
- Accession number :
- edsair.doi.dedup.....f25d7c7acbf1117a020d311aa68c381f