Back to Search
Start Over
Roles for Hedgehog signaling in androgen production and prostate ductal morphogenesis
- Source :
- Developmental Biology. 267:387-398
- Publication Year :
- 2004
- Publisher :
- Elsevier BV, 2004.
-
Abstract
- Previous studies have demonstrated that the Hedgehog (Hh) signaling pathway plays a critical role in the development and patterning of many endodermally derived tissues. We have investigated the role of Sonic hedgehog (Shh) in formation of the prostate gland by examining the urogenital phenotype of Shh mutant fetuses. Consistent with earlier work reporting an essential role for Shh in prostate induction, we have found that Shh mutant fetuses display abnormal urogenital development and fail to form prostate buds. Unexpectedly, however, we have discovered that this prostate defect could be rescued by three different methods: renal grafting, explant culture in the presence of androgens, and administration of dihydrotestosterone (DHT) to pregnant mice, indicating that the prostate defect in Shh mutants is due to insufficient levels of androgens. Furthermore, we find that the inhibition of Hh pathway signaling by treatment with cyclopamine does not block prostate formation in explant culture, but instead produces morphological defects consistent with a role for Hh signaling in ductal patterning. Taken together, our studies indicate that the initial organogenesis of the prostate proceeds independently of Shh, but that Shh or other Hh ligands may play a role in subsequent events that pattern the prostate.
- Subjects :
- Male
Organogenesis
Mice
chemistry.chemical_compound
Pregnancy
Prostate
Morphogenesis
Sonic hedgehog
In Situ Hybridization
Mice, Knockout
biology
Reverse Transcriptase Polymerase Chain Reaction
Endoderm
Veratrum Alkaloids
Gene Expression Regulation, Developmental
Dihydrotestosterone
Immunohistochemistry
Hedgehog signaling pathway
Cell biology
medicine.anatomical_structure
embryonic structures
Androgens
Female
Prostate induction
Triolein
Signal Transduction
medicine.drug
medicine.medical_specialty
animal structures
Cyclopamine
medicine.drug_class
Tissue recombination
Ductal morphogenesis
Internal medicine
medicine
Animals
Hedgehog Proteins
Molecular Biology
Hedgehog
DNA Primers
Cell Biology
Androgen
Endocrinology
chemistry
Trans-Activators
biology.protein
Developmental Biology
Subjects
Details
- ISSN :
- 00121606
- Volume :
- 267
- Database :
- OpenAIRE
- Journal :
- Developmental Biology
- Accession number :
- edsair.doi.dedup.....f25e4677444a1dcb4b755682c2edf9b8
- Full Text :
- https://doi.org/10.1016/j.ydbio.2003.11.018