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Immunohistochemical and genetic analysis of mandibular cysts in heterozygous ptc knockout mice
- Source :
- Journal of Oral Pathology and Medicine. 32:108-113
- Publication Year :
- 2003
- Publisher :
- Wiley, 2003.
-
Abstract
- Background: Alterations of human patched (ptc) homolog have been proven to be responsible for basal cell nevus syndrome (BCNS). Mandibular cysts in heterozygous ptc knockout mouse (ptc+/− mouse) were microradiologically, histologically, immunohistochemically, and genetically examined to investigate the possible role of the ptc gene and its associates in the jaw cysts. Methods: The mandibular bones were prepared from 63 ptc+/− mice and 6 ptc+/+ mice. Soft X-ray radiographs and histological sections were examined for detection of the presence of mandibular cysts. The mandibular cysts were immunohistochemically investigated using anti-ptc, shh, and smo antibodies. PCR analysis of loss of heterozygosity (LOH) of ptc was performed in genomic DNA from the mandibular cysts. Results: Six ptc+/+ mice showed no pathologic change in any examinations. Microradiologically, ptc+/− mice did not show any apparent lesion. Mandibular cysts were often multiple, and were histologically detected in the alveolar bones or periodontal ligaments of the molars in 16 (25.4%) ptc+/− mice. The mandibular cysts were lined by thin parakeratotic stratified squamous epithelium and contained keratinized materials. Immunohistochemical examination showed sonic hedgehog (shh) protein mainly in cyst lining epithelium, and ptc and smoothened (smo) proteins in cyst lining epithelium, and surrounding fibrous connective tissue. Expression of ptc protein in the cyst lining epithelium tended to be weak as compared with incisor enamel organs and gingival stratified squamous epithelium. LOH of the ptc gene couldn't be found in lining epithelium of mandibular cysts in any ptc+/− mice. Conclusions: Ptc+/− mouse is a useful model of BCNS from the standpoint of occurrence of jaw cysts, and downregulation of ptc protein in cyst lining epithelium caused by gene targeting would be associated with formation of jaw cysts in ptc+/− mice.
- Subjects :
- Male
Patched Receptors
Periodontium
Heterozygote
Cancer Research
medicine.medical_specialty
Pathology
endocrine system diseases
Basal Cell Nevus Syndrome
Gene Expression
Loss of Heterozygosity
Receptors, Cell Surface
Stratified squamous epithelium
Biology
Polymerase Chain Reaction
Receptors, G-Protein-Coupled
Pathology and Forensic Medicine
Mice
stomatognathic system
Odontogenic cyst
medicine
Animals
Hedgehog Proteins
Mandibular Diseases
Cyst
Mice, Knockout
Intracellular Signaling Peptides and Proteins
Mandible
Membrane Proteins
Tooth Germ
Anatomical pathology
medicine.disease
Immunohistochemistry
Smoothened Receptor
Epithelium
Mice, Inbred C57BL
Patched-1 Receptor
stomatognathic diseases
medicine.anatomical_structure
Otorhinolaryngology
Models, Animal
Odontogenic Cysts
Trans-Activators
Periodontics
Female
Oral Surgery
Subjects
Details
- ISSN :
- 16000714 and 09042512
- Volume :
- 32
- Database :
- OpenAIRE
- Journal :
- Journal of Oral Pathology and Medicine
- Accession number :
- edsair.doi.dedup.....f275f65065e14294a64aaed92cb6ef82