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Paper-based Microreactor Integrating Cell Culture and Subsequent Immunoassay for the Investigation of Cellular Phosphorylation
- Source :
- ACS Applied Materials & Interfaces. 6:22423-22429
- Publication Year :
- 2014
- Publisher :
- American Chemical Society (ACS), 2014.
-
Abstract
- Investigation of cellular phosphorylation and signaling pathway has recently gained much attention for the study of pathogenesis of cancer. Related conventional bioanalytical operations for this study including cell culture and Western blotting are time-consuming and labor-intensive. In this work, a paper-based microreactor has been developed to integrate cell culture and subsequent immunoassay on a single paper. The paper-based microreactor was a filter paper with an array of circular zones for running multiple cell cultures and subsequent immunoassays. Cancer cells were directly seeded in the circular zones without hydrogel encapsulation and cultured for 1 day. Subsequently, protein expressions including structural, functional, and phosphorylated proteins of the cells could be detected by their specific antibodies, respectively. Study of the activation level of phosphorylated Stat3 of liver cancer cells stimulated by IL-6 cytokine was demonstrated by the paper-based microreactor. This technique can highly reduce tedious bioanalytical operation and sample and reagent consumption. Also, the time required by the entire process can be shortened. This work provides a simple and rapid screening tool for the investigation of cellular phosphorylation and signaling pathway for understanding the pathogenesis of cancer. In addition, the operation of the paper-based microreactor is compatible to the molecular biological training, and therefore, it has the potential to be developed for routine protocol for various research areas in conventional bioanalytical laboratories.
- Subjects :
- Paper
Materials science
medicine.medical_treatment
Cell Culture Techniques
Biosensing Techniques
Sensitivity and Specificity
Cell Line, Tumor
medicine
Humans
General Materials Science
Immunoassay
medicine.diagnostic_test
Reproducibility of Results
Equipment Design
Neoplasms, Experimental
Microfluidic Analytical Techniques
Equipment Failure Analysis
Systems Integration
Blot
Cytokine
Biochemistry
Cell culture
Cancer cell
Cytokines
Phosphorylation
Microreactor
Signal transduction
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 6
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....f291cb324e0e8ff96de8600321f0f2a8