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Escherichia coli bactofection using Lipofectamine
- Source :
- Analytical biochemistry. 439(2)
- Publication Year :
- 2013
-
Abstract
- Successful gene delivery into mammalian cells using bactofection requires entry of the bacterial vector via cell surface integrin receptors followed by release of plasmid DNA into the cellular environment. We show, for the first time, that addition of the DNA transfection reagent Lipofectamine improves entry of invasive Escherichia coli into HeLa cells and enhances up to 2.8-fold green fluorescent protein (GFP) expression from a reporter plasmid. The addition of Lipofectamine may be applicable to other bacterial vectors to increase their DNA delivery efficiency into mammalian cells.
- Subjects :
- Cell
Green Fluorescent Proteins
Biophysics
Gene delivery
medicine.disease_cause
Transfection
Biochemistry
Green fluorescent protein
HeLa
Plasmid
Genes, Reporter
medicine
Escherichia coli
Humans
Vector (molecular biology)
Molecular Biology
biology
Cell Biology
biology.organism_classification
Molecular biology
Lipids
medicine.anatomical_structure
Gene Expression Regulation
Lipofectamine
HeLa Cells
Plasmids
Subjects
Details
- ISSN :
- 10960309
- Volume :
- 439
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Analytical biochemistry
- Accession number :
- edsair.doi.dedup.....f2c030399b363e92695fc48610d4f718