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A Scalable 'Junction Substrate' to Engineer Robust DNA Circuits
- Source :
- Journal of the American Chemical Society. 140:9979-9985
- Publication Year :
- 2018
- Publisher :
- American Chemical Society (ACS), 2018.
-
Abstract
- Versatile building blocks are essential for building complex and scaled-up DNA circuits. In this study, we propose a conceptually new scalable architecture called a "junction substrate" (J-substrate) that is linked by prepurified double-stranded DNA molecules. As a proof-of-concept, this novel type of substrate has been utilized to build multi-input DNA circuits, offering several advantages over the conventional substrate (referred to as a "linear substrate", L-substrate). First, the J-substrate does not require long DNA strands, thus avoiding significant synthetic errors and costs. Second, the traditional PAGE purification method is technically facilitated to obtain high-purity substrates, whereby the initial leakage is effectively eliminated. Third, the asymptotic leakage is eliminated by introducing the "junction". Finally, circuits with the optimized J-substrate architecture exhibit fast kinetics. We believe that the proposed architecture constitutes a sophisticated chassis for constructing complex circuits.
- Subjects :
- Chassis
Hardware_PERFORMANCEANDRELIABILITY
010402 general chemistry
Proof of Concept Study
01 natural sciences
Biochemistry
Catalysis
chemistry.chemical_compound
Colloid and Surface Chemistry
Hardware_INTEGRATEDCIRCUITS
Purification methods
Leakage (electronics)
Electronic circuit
010405 organic chemistry
Chemistry
business.industry
DNA
General Chemistry
Scalable architecture
0104 chemical sciences
Proof of concept
Scalability
Electrophoresis, Polyacrylamide Gel
business
Computer hardware
Subjects
Details
- ISSN :
- 15205126 and 00027863
- Volume :
- 140
- Database :
- OpenAIRE
- Journal :
- Journal of the American Chemical Society
- Accession number :
- edsair.doi.dedup.....f2c79a2e3dcb4d7802d895c2f699bd82