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Binding site of MraZ transcription factor in Mollicutes
- Source :
- Biochimie. 125:59-65
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Mollicutes (mycoplasmas) feature a significant loss of known regulators of gene expression. Here, we identified the recognition site of the MraZ-family regulator of Mycoplasma gallisepticum, which is conserved in many species of different clades within class Mollicutes. The MraZ binding site is AAAGTG[T/G], in the promoter of mraZ gene it forms a series of direct repeats with a structure (AAAGTG[T/G]N3)k, where k = 3 most frequently. MraZ binds to a single repeat as an octamer complex. MraZ can also bind a single binding site or a series of repeats with different spacer lengths (2–4 nt); thus, it may play a role in the regulation of multiple operons in Mollicutes. In M. gallisepticum, MraZ acts as a transcriptional activator. The overexpression of MraZ leads to moderate filamentation of cells and the formation of aggregates, likely as a result of incomplete cytokinesis.
- Subjects :
- 0301 basic medicine
Mycoplasma gallisepticum
Transcription, Genetic
Operon
030106 microbiology
Mollicutes
Response Elements
Biochemistry
03 medical and health sciences
Mycoplasma
Bacterial Proteins
Transcription (biology)
Direct repeat
Histone octamer
Binding site
Gene
Genetics
biology
General Medicine
biology.organism_classification
Molecular biology
030104 developmental biology
Transcription factor
Transcription
Transcription Factors
Subjects
Details
- ISSN :
- 03009084
- Volume :
- 125
- Database :
- OpenAIRE
- Journal :
- Biochimie
- Accession number :
- edsair.doi.dedup.....f2ee43f8f45776a8e13c13ca4a08d416