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Inhabiting plant roots, nematodes, and truffles—polyphilus, a new helotialean genus with two globally distributed species

Authors :
Damien Blaudez
Michel Chalot
Imre Zagyva
Samad Ashrafi
Gábor M. Kovács
Abdelfattah A. Dababat
Jose G. Maciá-Vicente
Dániel G. Knapp
Wolfgang Maier
Plant Protect Serv
Wageningen University and Research [Wageningen] (WUR)
Laboratoire Interdisciplinaire des Environnements Continentaux (LIEC)
Institut national des sciences de l'Univers (INSU - CNRS)-Observatoire Terre et Environnement de Lorraine (OTELo)
Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut national des sciences de l'Univers (INSU - CNRS)-Université de Lorraine (UL)-Centre National de la Recherche Scientifique (CNRS)-Institut Ecologie et Environnement (INEE)
Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)
Laboratoire Chrono-environnement - CNRS - UBFC (UMR 6249) (LCE)
Centre National de la Recherche Scientifique (CNRS)-Université de Franche-Comté (UFC)
Université Bourgogne Franche-Comté [COMUE] (UBFC)-Université Bourgogne Franche-Comté [COMUE] (UBFC)
Department of Psychiatry and Psychotherapy
Rheinische Friedrich-Wilhelms-Universität Bonn
Max-Planck-Institut für Sonnensystemforschung (MPS)
Max-Planck-Gesellschaft
Source :
Mycologia, Mycologia, Mycological Society of America, 2018, 110 (2), pp.286-299. ⟨10.1080/00275514.2018.1448167⟩, Mycologia 110 (2018) 2, Mycologia, 110(2), 286-299
Publication Year :
2018

Abstract

Fungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study of DSE fungi of semiarid grasslands, from several other sites, and collected recently from a pezizalean truffle ascoma and eggs of the cereal cyst nematode Heterodera filipjevi. The taxonomic positions and phylogenetic relationships of 21 isolates with different hosts and geographic origins were studied in detail. Four loci, namely, nuc rDNA ITS1-5.8S-ITS2 (internal transcribed spacer [ITS]), partial 28S nuc rDNA (28S), partial 18S nuc rDNA (18S), and partial RNA polymerase II second-largest subunit (RPB2), were amplified and sequenced for molecular phylogenetic analyses. Analyses of similar ITS sequences from public databases revealed two globally distributed lineages detected in several biomes from different geographic regions. The host interaction of isolates from nematodes was examined using in vitro bioassays, which revealed that the fungi could penetrate nematode cysts and colonize eggs of H. filipjevi, confirming observations from field-collected samples. This is the first report of a DSE, and we are not aware of other helotialean fungal species colonizing the eggs of a plant-parasitic nematode. Neither conidiomata and conidia nor ascomata formation was detected in any of the isolates. Based on molecular phylogenetic analyses, these isolates represent a distinct lineage within the Helotiales in the Hyaloscyphaceae. For this lineage, we propose here the new genus Polyphilus represented by two new species, P. sieberi and P. frankenii.

Details

Language :
English
ISSN :
00275514
Volume :
110
Issue :
2
Database :
OpenAIRE
Journal :
Mycologia
Accession number :
edsair.doi.dedup.....f30c633d3759428aa22e341fb9df7978
Full Text :
https://doi.org/10.1080/00275514.2018.1448167