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Results of a collaborative study on DNA identification of aged bone samples

Authors :
Judith Zander
Marie Allen
Joanna Vella
Petra Lotz
Ruth Galdies
Alain Stevanovitch
Olivier Cepil
Marion Nagy
Daniel Vanek
Wafa Ali Rashid Al Tayyari
Dina Al Salafi
Hicham El Ossmani
Mishel Stephenson
Jindrich Novotny
Jan Cemper-Kiesslich
Jan Frolík
Jitka Dubska-Votrubova
Greet De Cock
Michel Bottinelli
Francois Guidet
Marguerethe Stenersen
Juliane Strien
Wendy Arguetaa
Magdalena M. Bus
Angie Ambers
Anna Hoefges
Cristian Bogdan Iancu
D.R. Sumita
Ahmed Anwar Al Afeefi
Alessandro Maresca
Hajar Rachid
Jessica Rothe
Bruce Budowle
Martin Pospíšek
Stijn Desmyter
Hamid El Amri
Khudooma Saeed Al Naimi
Khalid Ismaeil Al Hosani
Sebastian Grün
Source :
Croatian Medical Journal, Volume 58, Issue 3
Publication Year :
2017
Publisher :
Medicinska naklada, 2017.

Abstract

Aim A collaborative exercise with several institutes was organized by the Forensic DNA Service (FDNAS) and the Institute of the Legal Medicine, 2nd Faculty of Medicine, Charles University in Prague, Czech Republic, with the aim to test performance of different laboratories carrying out DNA analysis of relatively old bone samples. Methods Eighteen laboratories participating in the collaborative exercise were asked to perform DNA typing of two samples of bone powder. Two bone samples provided by the National Museum and the Institute of Archaelogy in Prague, Czech Republic, came from archeological excavations and were estimated to be approximately 150 and 400 years old. The methods of genetic characterization including autosomal, gonosomal, and mitochondrial markers was selected solely at the discretion of the participating laboratory. Results Although the participating laboratories used different extraction and amplification strategies, concordant results were obtained from the relatively intact 150 years old bone sample. Typing was more problematic with the analysis of the 400 years old bone sample due to poorer quality. Conclusion The laboratories performing identification DNA analysis of bone and teeth samples should regularly test their ability to correctly perform DNA-based identification on bone samples containing degraded DNA and potential inhibitors and demonstrate that risk of contamination is minimized.

Details

Language :
English
ISSN :
13328166 and 03539504
Volume :
58
Issue :
3
Database :
OpenAIRE
Journal :
Croatian Medical Journal
Accession number :
edsair.doi.dedup.....f333e93e06c37c4b33e8708afbf7bcc6