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Analysis of Semen Parameters in α1,3-Galactosyltransferase-/- Boars

Authors :
Sun-A Ock
Gi-Sun Im
Sung-Woo Kim
Hyeon Jong Yang
Mi-Ryung Park
Dae-Jin Kwon
Jae-Seok Woo
In-Sul Hwang
Keon Bong Oh
Seung-Chan Lee
Seongsoo Hwang
Source :
Journal of Animal Reproduction and Biotechnology, Vol 32, Iss 2, Pp 53-58 (2017)
Publication Year :
2017
Publisher :
The Korean Society of Animal Reproduction and Biotechnology, 2017.

Abstract

It is very difficult to get the information about semen quality analysis in transgenic pigs because of limited numbers and research facilities. Therefore, in the present study, we analyzed the semen quality of transgenic boars generated for xenotransplantation research. Briefly, the semen samples were collected from 5 homozygous α1,3-Galactosyltransferase knock-out (GalT-/-) transgenic boars and immediately transported to the laboratory. These semen samples were decupled with DPBS and conducted to analyze semen parameters by a computer-assisted semen analysis (CASA) system. The boar semen were examined all 12 parameters such as total motility (TM), curvilinear velocity (VCL), straight line velocity (VSL), average path velocity (VAP), and hyperactivated (HYP), etc. In results, among the 5 GalT-/- boars, three boars (#134, 144, and 170) showed normal range of semen parameters, but #199 and 171 boars showed abnormal ranges of semen parameters according to standard ranges of semen parameters. Unfortunately, #171 boar showed azoospermia symptom with rare sperm counts in the original semen. Conclusively, assessment of semen parameters by CASA system is useful to pre-screening of reproductively healthy boar prior to natural mating and artificial insemination for multiplication and breeding.

Details

Language :
English
ISSN :
26714663 and 26714639
Volume :
32
Issue :
2
Database :
OpenAIRE
Journal :
Journal of Animal Reproduction and Biotechnology
Accession number :
edsair.doi.dedup.....f33fd43762ffc8c3a2c52a8300aefb0e