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Evaluation of two TaqMan PCR assays for the detection of HIV-1 proviral DNA in blood samples
- Source :
- Journal of Microbiological Methods. 65:350-353
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- This study compared two published TaqMan PCR assays targeting different regions of the HIV-1 genome for detection of HIV-1 proviral DNA. The gag specific PCR demonstrated a lower sensitivity than the assay targeting the LTR region. The LTR assay is a highly reproducible and specific technique for HIV-1 proviral DNA detection.
- Subjects :
- Microbiology (medical)
viruses
Pcr assay
Human immunodeficiency virus (HIV)
HIV Infections
Proviral dna
Biology
medicine.disease_cause
Polymerase Chain Reaction
Sensitivity and Specificity
Microbiology
Genome
Proviruses
medicine
TaqMan
Humans
Taq Polymerase
Molecular Biology
HIV Long Terminal Repeat
Reproducibility of Results
virus diseases
Genes, gag
Virology
Molecular biology
DNA, Viral
HIV-1
Subjects
Details
- ISSN :
- 01677012
- Volume :
- 65
- Database :
- OpenAIRE
- Journal :
- Journal of Microbiological Methods
- Accession number :
- edsair.doi.dedup.....f345ad35a059767dbe60f2e2c672ba72
- Full Text :
- https://doi.org/10.1016/j.mimet.2005.08.003