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Establishment of a novel MALT lymphoma cell line, ma-1, from a patient with t(14;18)(q32;q21)-positive Helicobacter Pylori-Independent Gastric MALT Lymphoma
- Source :
- Genes, Chromosomes and Cancer. 50:908-921
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- Although t(11;18)(q21;q21), t(1;14)(p22;q32), and a few other genetic mutations are specific markers for the Helicobacter pylori (HP)-independent status of gastric mucosa-associated lymphoid tissue (MALT) lymphoma, the molecular mechanisms responsible for HP-independence of gastric MALT lymphoma without such translocations and mutations remain uncharacterized. In the present study, we describe the establishment and characterization of a novel MALT lymphoma cell line, MA-1, which was derived from a gastric MALT lymphoma which was negative for both t(11;18)(q21;q21) and t(1;14)(p22;q32); the patient had failed HP eradication therapy and chemotherapy. The cell morphology and the immunophenotype of this cell line were similar to that of the original gastric MALT lymphoma. Comparative genomic hybridization analysis showed no significant gene copy number changes. Spectral karyotyping displayed a near-diploid chromosome content (482nXY), with at least 13 chromosome structural abnormalities. Furthermore, fluorescence in situ hybridization analyses disclosed the existence of three sub-clones, characterized by t(14;18)(q32;q21)/IGH-BCL2, t(14;18)(q32;q21)/IGH-MALT1, and the presence of both chromosomal translocations in the same cell, respectively; whereas amplification of the genes CRAD9, TRAF2, and BCL10 were not found. In conclusion, we have established the first human gastric MALT lymphoma cell line, which is characterized by unusual and complex chromosome translocations and will be useful to explore further the molecular mechanisms of HP-independence in gastric MALT lymphoma.
- Subjects :
- Male
Cancer Research
Gene Dosage
Biology
Cell morphology
Translocation, Genetic
Helicobacter Infections
Immunophenotyping
Stomach Neoplasms
immune system diseases
Cell Line, Tumor
hemic and lymphatic diseases
Genetics
medicine
Humans
In Situ Hybridization, Fluorescence
B cell
Chromosomes, Human, Pair 14
Comparative Genomic Hybridization
Helicobacter pylori
medicine.diagnostic_test
Lymphoma, Non-Hodgkin
Spectral Karyotyping
MALT lymphoma
Lymphoma, B-Cell, Marginal Zone
Middle Aged
Flow Cytometry
medicine.disease
biology.organism_classification
Immunohistochemistry
Molecular biology
digestive system diseases
BCL10
Lymphoma
medicine.anatomical_structure
Immunology
Chromosomes, Human, Pair 18
Microsatellite Repeats
Comparative genomic hybridization
Fluorescence in situ hybridization
Subjects
Details
- ISSN :
- 10452257
- Volume :
- 50
- Database :
- OpenAIRE
- Journal :
- Genes, Chromosomes and Cancer
- Accession number :
- edsair.doi.dedup.....f34eff59ac455e881c357ae6bb36f510