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A Highly Sensitive Oligonucleotide Hybridization Assay for Klebsiella pneumoniae Carbapenemase with the Probes on a Gold Nanoparticles Modified Glassy Carbon Electrode
- Source :
- Journal of AOAC INTERNATIONAL. 98:1728-1732
- Publication Year :
- 2015
- Publisher :
- Oxford University Press (OUP), 2015.
-
Abstract
- To develop a new electrochemical DNA biosensor for determination of Klebsiella pneumoniae carbapenemase, a highly sensitive and selective electrochemical biosensor for DNA detection was constructed based on a glassy carbon electrode (GCE) modified with gold nanoparticles (Au-nano). The Au-nano/GCE was characterized by scanning electromicroscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The hybridization detection was measured by differential pulse voltammetry using methylene blue as the hybridization indicator. The dynamic range of detection of the sensor for the target DNA sequences was from 1 × 10–11 to 1 × 10–8 M, with an LOD of 1 × 10–12 M. The DNA biosensor had excellent specificity for distinguishing complementary DNA sequence in the presence of non-complementary and mismatched DNA sequence. The Au-nano/GCE showed significant improvement in electrochemical characteristics, and this biosensor was successfully applied for determination of K. pneumoniae.
- Subjects :
- Oligonucleotides
Analytical chemistry
Metal Nanoparticles
Biosensing Techniques
beta-Lactamases
Analytical Chemistry
Nucleic acid thermodynamics
chemistry.chemical_compound
Bacterial Proteins
Electrochemistry
Environmental Chemistry
Electrodes
Pharmacology
Chemistry
Oligonucleotide
technology, industry, and agriculture
Nucleic Acid Hybridization
Carbon
Dielectric spectroscopy
Klebsiella pneumoniae
Colloidal gold
Gold
Differential pulse voltammetry
Cyclic voltammetry
Agronomy and Crop Science
Biosensor
DNA
Food Science
Nuclear chemistry
Subjects
Details
- ISSN :
- 19447922 and 10603271
- Volume :
- 98
- Database :
- OpenAIRE
- Journal :
- Journal of AOAC INTERNATIONAL
- Accession number :
- edsair.doi.dedup.....f3740430fbea72082bf34cd5caeee7ea