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Pyrogenic synthetic amorphous silica (NM-203): Genotoxicity in rats following sub-chronic oral exposure
- Source :
- Mutation Research-Genetic Toxicology and Environmental Mutagenesis, Mutation Research-Genetic Toxicology and Environmental Mutagenesis, 2022, 876-877, pp.503458. ⟨10.1016/j.mrgentox.2022.503458⟩
- Publication Year :
- 2022
- Publisher :
- Elsevier BV, 2022.
-
Abstract
- International audience; The genotoxicity of nano-structured synthetic amorphous silica (SAS), a common food additive, was investigated in vivo in rats. A 90-day oral toxicity study was performed according to OECD test guideline 408 and the genotoxicity of pyrogenic SAS nanomaterial NM-203 was assessed in several organs, using complementary tests. Adult Sprague-Dawley rats of both sexes were treated orally for 90 days with 0, 2, 5, 10, 20, or 50 mg SAS/kg bw per day. Dose levels were selected to approximate expected human dietary exposures to SAS. DNA strand breaks were evaluated by the comet assay in blood, bone marrow, liver, and spleen according to OECD test guideline 489; mutations induced in bone marrow precursors of erythrocytes were assessed by the Pig-a assay and chromosome/ genome damage by the micronucleus assay in blood (OECD test guideline 474) and colon. No treatment-related increases of gene (Pig-a) or chromosome/genome (micronucleus) mutations were detected in the blood. The percentage of micronucleated cells was not increased in the colon of treated rats. Among the organs analyzed by the comet assay, the spleen was the only target showing a weak but biologically relevant genotoxic effect.
- Subjects :
- Male
Health, Toxicology and Mutagenesis
MESH: Mutagenicity Tests
nanoparticule
Rats, Sprague-Dawley
comet assay
Genetics
Animals
toxicité
rat
Synthetic amorphous silica
silice synthétique amorphe
food additive
MESH: DNA Damage
silicon dioxide
Micronucleus Tests
nanoparticle
additif
genotoxicity
toxicity
toxicologie
test des comètes
Rats
dioxine de silicone
micronucleus test
sécruité des aliments
[SDV.TOX]Life Sciences [q-bio]/Toxicology
génotoxicité
DNA damage
Female
test du micronucleus
MESH: Nanoparticles
toxicology
Subjects
Details
- ISSN :
- 13835718
- Database :
- OpenAIRE
- Journal :
- Mutation Research/Genetic Toxicology and Environmental Mutagenesis
- Accession number :
- edsair.doi.dedup.....f37ae3714f5a4ed9c1b7f99b4d0487a5