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MiR-1260b protects against LPS-induced degenerative changes in nucleus pulposus cells through targeting TCF7L2

Authors :
Shijie, Chen
Guixia, Shi
Jin, Zeng
Ping Huang, Li
Yi, Peng
Zhiyu, Ding
Hong Qing, Cao
Ruping, Zheng
Weiguo, Wang
Source :
Human Cell. 35:779-791
Publication Year :
2022
Publisher :
Springer Science and Business Media LLC, 2022.

Abstract

Nucleus pulposus (NP) cells play a critical role in maintaining intervertebral disc integrity through producing the components of extracellular matrix (ECM). NP cell dysfunction, including senescence and hyper-apoptosis, has been regarded as critical events during intervertebral disc degeneration development. In the present study, we found that Transcription Factor 7-Like 2 (TCF7L2) was overexpressed within degenerative intervertebral disc tissue samples, and TCF7L2 silencing improved lipopolysaccharide (LPS)-induced repression on NP cell proliferation, ECM synthesis, and LPS-induced NP cell senescence. miR-1260b directly targeted TCF7L2 and inhibited TCF7L2 expression. miR-1260b overexpression improved LPS-induced degenerative changes in NP cells; more importantly, TCF7L2 overexpression significantly reversed the effects of miR-1260b overexpression on LPS-stimulated degenerative changes within NP cells. For the first time, we demonstrated the function of the miR-1260b/TCF7L2 axis on the phenotypic maintenance of chondrocyte-like NP cells and ECM synthesis by NP cells under LPS stimulation.

Details

ISSN :
17490774
Volume :
35
Database :
OpenAIRE
Journal :
Human Cell
Accession number :
edsair.doi.dedup.....f3d385a43f1c908363f62ba38787087d