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A human tissue map of 5-hydroxymethylcytosines exhibits tissue specificity through gene and enhancer modulation
- Source :
- Nature Communications, Vol 11, Iss 1, Pp 1-11 (2020), Nature Communications
- Publication Year :
- 2020
- Publisher :
- Nature Portfolio, 2020.
-
Abstract
- DNA 5-hydroxymethylcytosine (5hmC) modification is known to be associated with gene transcription and frequently used as a mark to investigate dynamic DNA methylation conversion during mammalian development and in human diseases. However, the lack of genome-wide 5hmC profiles in different human tissue types impedes drawing generalized conclusions about how 5hmC is implicated in transcription activity and tissue specificity. To meet this need, we describe the development of a 5hmC tissue map by characterizing the genomic distributions of 5hmC in 19 human tissues derived from ten organ systems. Subsequent sequencing results enabled the identification of genome-wide 5hmC distributions that uniquely separates samples by tissue type. Further comparison of the 5hmC profiles with transcriptomes and histone modifications revealed that 5hmC is preferentially enriched on tissue-specific gene bodies and enhancers. Taken together, the results provide an extensive 5hmC map across diverse human tissue types that suggests a potential role of 5hmC in tissue-specific development; as well as a resource to facilitate future studies of DNA demethylation in pathogenesis and the development of 5hmC as biomarkers.<br />DNA 5-hydroxymethylcytosine (5hmC) modification is associated with gene transcription and used as a mark of mammalian development. Here the authors report a comprehensive 5hmC tissue map and analysis of 5hmC genomic distributions in 19 human tissues derived from 10 organ systems, thus providing insights into the role of 5hmC in tissue-specific development.
- Subjects :
- Epigenomics
Transcriptional Activation
0301 basic medicine
Science
General Physics and Astronomy
Computational biology
Article
General Biochemistry, Genetics and Molecular Biology
Epigenesis, Genetic
Histones
Cytosine
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Transcription (biology)
DNA metabolism
Humans
Enhancer
Gene
DNA methylation
Multidisciplinary
biology
Genome, Human
Chromosome Mapping
DNA
General Chemistry
Enhancer Elements, Genetic
030104 developmental biology
Histone
DNA demethylation
chemistry
Organ Specificity
030220 oncology & carcinogenesis
5-Methylcytosine
biology.protein
CpG Islands
Transcription Factors
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 11
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- Nature Communications
- Accession number :
- edsair.doi.dedup.....f3e26e4dd9e5584cbcb5a64a4d7fd47d