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Expression and cell membrane localization of rat M3 muscarinic acetylcholine receptor produced in Sf 9 insect cells using the baculovirus system
- Source :
- FEBS Letters. 283:52-56
- Publication Year :
- 1991
- Publisher :
- Wiley, 1991.
-
Abstract
- A recombinant baculovirus bearing the cDNA coding for the rat muscarinic acetylcholine receptor subtype M3 placed under the control of the Autographa californica nuclear polyhedrosis virus polyhedrin gene promoter, was constructed. Polymerase chain reaction screening was used to identify the recombinant baculovirus. Northern blot analysis of total RNA from insect cells infected with the recombinant baculovirus indicated that the transcripts were abundant. Binding assays carried out with the muscarinic antagonist [3H]quinuclidinyl benzilate indicated that more than 1 × 106 receptors were produced per cell. Immunofluorescence microscopy showed that the receptor is located on the cell surface.
- Subjects :
- Gene Expression Regulation, Viral
Insecta
Genes, Viral
viruses
Genetic Vectors
Molecular Sequence Data
Immunofluorescence
Biophysics
Biology
Polymerase Chain Reaction
Biochemistry
law.invention
Baculovirus expression
Structural Biology
law
Muscarinic acetylcholine receptor
Genetics
medicine
Polyhedrin
Animals
Receptor
Molecular Biology
Acetylcholine receptor
Base Sequence
Cell Membrane
Muscarinic receptor
fungi
Muscarinic antagonist
Muscarinic acetylcholine receptor M3
DNA
Cell Biology
Blotting, Northern
Receptors, Muscarinic
Molecular biology
Acetylcholine
Rats
Quinuclidinyl Benzilate
Microscopy, Fluorescence
Recombinant DNA
Autoradiography
RNA, Viral
Baculoviridae
PCR screening
medicine.drug
Subjects
Details
- ISSN :
- 00145793
- Volume :
- 283
- Database :
- OpenAIRE
- Journal :
- FEBS Letters
- Accession number :
- edsair.doi.dedup.....f3e47aa3f182a4fa28de60686407df12