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Bone Marrow Protein Oxidation in Response to Ionizing Radiation in C57BL/6J Mice
- Source :
- Proteomes, Proteomes, Vol 2, Iss 3, Pp 291-302 (2014), Proteomes; Volume 2; Issue 3; Pages: 291-302
- Publication Year :
- 2014
- Publisher :
- MDPI, 2014.
-
Abstract
- The bone marrow is one of the most radio-sensitive tissues. Accidental ionizing radiation exposure can damage mature blood cells and hematopoietic progenitor/stem cells, and mortality can result from hematopoietic insufficiency and infection. Ionizing radiation induces alterations in gene and protein expression in hematopoietic tissue. Here we investigated radiation effects on protein carbonylation, a primary marker for protein oxidative damage. C57BL/6 mice were either sham irradiated or exposed to 7.5 Gy 60Co (0.6 Gy/min) total body irradiation. Bone marrow was obtained 24 h post-irradiation. Two dimensional (2-D) gel electrophoresis and Oxyblot immunodetection were used to discover carbonylated proteins, and peptide mass fingerprinting was performed for identification. 2D gels allowed the detection of 13 carbonylated proteins in the bone marrow; seven of these were identified, with two pairs of the same protein. Baseline levels of carbonylation were found in 78 kDa glucose-related protein, heat shock protein cognate 71 KDa, actin, chitinase-like protein 3 (CHI3L1), and carbonic anhydrase 2 (CAII). Radiation increased carbonylation in four proteins, including CHI3L1 and CAII, and induced carbonylation of one additional protein (not identified). Our findings indicate that the profile of specific protein carbonylation in bone marrow is substantially altered by ionizing radiation. Accordingly, protein oxidation may be a mechanism for reduced cell viability.
- Subjects :
- Protein Carbonylation
acute radiation syndrome
protein carbonylation
proteomic analysis
Clinical Biochemistry
Hematopoietic Tissue
lcsh:QR1-502
Total body irradiation
Biology
Protein oxidation
Biochemistry
Molecular biology
lcsh:Microbiology
Article
medicine.anatomical_structure
Peptide mass fingerprinting
Structural Biology
Heat shock protein
medicine
Bone marrow
Stem cell
Molecular Biology
Subjects
Details
- Language :
- English
- ISSN :
- 22277382
- Volume :
- 2
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Proteomes
- Accession number :
- edsair.doi.dedup.....f3e5f8f467bd0f0d3c2f275f212eb46a