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Intracellular dynamics of polydnavirus innexin homologues
- Source :
- Insect molecular biologyReferences. 29(5)
- Publication Year :
- 2019
-
Abstract
- Polydnaviruses associated with ichneumonid parasitoid wasps (Ichnoviruses) encode large numbers of genes, often in multigene families. The Ichnovirus Vinnexin gene family, which is expressed in parasitized lepidopteran larvae, encodes homologues of Innexins, the structural components of insect gap junctions. Here, we have examined intracellular behaviours of the Campoletis sonorensis Ichnovirus (CsIV) Vinnexins, alone and in combination with a host Innexin orthologue, Innexin2 (Inx2). QRT-PCR verified that transcription of CsIV vinnexins occurs contemporaneously with inx2, implying co-occurrence of Vinnexin and Inx2 proteins. Confocal microscopy demonstrated that epitope-tagged VinnexinG (VnxG) and VinnexinQ2 (VnxQ2) exhibit similar subcellular localization as Spodoptera frugiperda Inx2 (Sf-Inx2). Surface biotinylation assays verified that all three proteins localize to the cell surface, and cytochalasin B and nocodazole that they rely on actin and microtubule cytoskeletal networks for localization. Immunomicroscopy following co-transfection of constructs indicates extensive co-localization of Vinnexins with each other and Sf-Inx2, and live-cell imaging of mCherry-labelled Inx2 supports that Vinnexins may affect Sf-Inx2 distribution in a Vinnexin-specific fashion. Our findings support that the Vinnexins may disrupt host cell physiology in a protein-specific manner through altering gap junctional intercellular channel communication, as well as indirectly by affecting multicellular junction characteristics.
- Subjects :
- 0106 biological sciences
0301 basic medicine
food.ingredient
Genes, Viral
Transcription, Genetic
Wasps
Genes, Insect
Innexin
Biology
Spodoptera
01 natural sciences
03 medical and health sciences
chemistry.chemical_compound
food
Genetics
Gene family
Animals
Cytoskeleton
Molecular Biology
Actin
Polydnavirus
fungi
biology.organism_classification
Cell biology
010602 entomology
Nocodazole
030104 developmental biology
chemistry
Polydnaviridae
Insect Science
Larva
Multigene Family
Host-Pathogen Interactions
Ichnovirus
Subjects
Details
- ISSN :
- 13652583
- Volume :
- 29
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Insect molecular biologyReferences
- Accession number :
- edsair.doi.dedup.....f3ea344ed7fec15a19512f652ed33c4d