Back to Search Start Over

The Molecular Karyotype of 25 Clinical-Grade Human Embryonic Stem Cell Lines

Authors :
Paul A. De Sousa
Susan J. Kimber
Tilo Kunath
Zoe Hewitt
Amy Van Deusen
Maurice A. Canham
Liani Devito
J.M. Downie
Dusko Ilic
Harry Moore
Helen C. Murray
Daniel R. Brison
Source :
Scientific Reports, Canham, M A, Van Deusen, A, Brison, D R, De Sousa, P A, Downie, J, Devito, L, Hewitt, Z A, Ilic, D, Kimber, S J, Moore, H D, Murray, H & Kunath, T 2015, ' The molecular karyotype of 25 clinical-grade human embryonic stem cell lines ', Scientific Reports, vol. 5, 17258 . https://doi.org/10.1038/srep17258
Publication Year :
2015
Publisher :
Nature Publishing Group, 2015.

Abstract

The application of human embryonic stem cell (hESC) derivatives to regenerative medicine is now becoming a reality. Although the vast majority of hESC lines have been derived for research purposes only, about 50 lines have been established under Good Manufacturing Practice (GMP) conditions. Cell types differentiated from these designated lines may be used as a cell therapy to treat macular degeneration, Parkinson’s, Huntington’s, diabetes, osteoarthritis and other degenerative conditions. It is essential to know the genetic stability of the hESC lines before progressing to clinical trials. We evaluated the molecular karyotype of 25 clinical-grade hESC lines by whole-genome single nucleotide polymorphism (SNP) array analysis. A total of 15 unique copy number variations (CNVs) greater than 100 kb were detected, most of which were found to be naturally occurring in the human population and none were associated with culture adaptation. In addition, three copy-neutral loss of heterozygosity (CN-LOH) regions greater than 1 Mb were observed and all were relatively small and interstitial suggesting they did not arise in culture. The large number of available clinical-grade hESC lines with defined molecular karyotypes provides a substantial starting platform from which the development of pre-clinical and clinical trials in regenerative medicine can be realised.

Details

Language :
English
ISSN :
20452322
Volume :
5
Database :
OpenAIRE
Journal :
Scientific Reports
Accession number :
edsair.doi.dedup.....f49597ce6e3bf915bcdf23d89e2d900f
Full Text :
https://doi.org/10.1038/srep17258