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Stitching together the multiple dimensions of autophagy using metabolomics and transcriptomics reveals impacts on metabolism, development, and plant responses to the environment in arabidopsis
- Source :
- Plant Cell, Plant Cell, American Society of Plant Biologists, 2014, 26, pp.1857-1877. ⟨10.1105/tpc.114.124677⟩, The Plant cell, The Plant cell, American Society of Plant Biologists (ASPB), 2014, 26, pp.1857-1877. ⟨10.1105/tpc.114.124677⟩
- Publication Year :
- 2014
- Publisher :
- HAL CCSD, 2014.
-
Abstract
- Autophagy is a fundamental process in the plant life story, playing a key role in immunity, senescence, nutrient recycling, and adaptation to the environment. Transcriptomics and metabolomics of the rosette leaves of Arabidopsis thaliana autophagy mutants (atg) show that autophagy is essential for cell homeostasis and stress responses and that several metabolic pathways are affected. Depletion of hexoses, quercetins, and anthocyanins parallel the overaccumulation of several amino acids and related compounds, such as glutamate, methionine, glutathione, pipecolate, and 2-aminoadipate. Transcriptomic data show that the pathways for glutathione, methionine, raffinose, galacturonate, and anthocyanin are perturbed. Anthocyanin depletion in atg mutants, which was previously reported as a possible defect in flavonoid trafficking to the vacuole, appears due to the downregulation of the master genes encoding the enzymes and regulatory proteins involved in flavonoid biosynthesis. Overexpression of the PRODUCTION OF ANTHOCYANIN PIGMENT1 transcription factor restores anthocyanin accumulation in vacuoles of atg mutants. Transcriptome analyses reveal connections between autophagy and (1) salicylic acid biosynthesis and response, (2) cytokinin perception, (3) oxidative stress and plant defense, and possible interactions between autophagy and the COP9 signalosome machinery. The metabolic and transcriptomic signatures identified for the autophagy mutants are discussed and show consistencies with the observed phenotypes.
- Subjects :
- 2. Zero hunger
plant responses to the environment
autophagy
biology
[SDV]Life Sciences [q-bio]
Autophagy
fungi
food and beverages
Cell Biology
Plant Science
Vacuole
biology.organism_classification
Cell biology
Metabolic pathway
arabidopsis
Flavonoid biosynthesis
Biochemistry
Arabidopsis
Plant defense against herbivory
Large-Scale Biology Article
COP9 signalosome
Functional genomics
transcriptome
metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 10404651 and 1532298X
- Database :
- OpenAIRE
- Journal :
- Plant Cell, Plant Cell, American Society of Plant Biologists, 2014, 26, pp.1857-1877. ⟨10.1105/tpc.114.124677⟩, The Plant cell, The Plant cell, American Society of Plant Biologists (ASPB), 2014, 26, pp.1857-1877. ⟨10.1105/tpc.114.124677⟩
- Accession number :
- edsair.doi.dedup.....f49f94bc95eb3ab46919d6b85305e728