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Sanggenon C induces apoptosis of colon cancer cells via inhibition of NO production, iNOS expression and ROS activation of the mitochondrial pathway
- Source :
- Oncology Reports
- Publication Year :
- 2017
- Publisher :
- D.A. Spandidos, 2017.
-
Abstract
- Sanggenon C is a well-known, major active agent of the flavonoid derivative of benzopyrone with valuable biological properties, including anticancer, anti-inflammatory, antimicrobial, antiviral, antithrombotic, and immune-modulatory activities. In this study, we investigated the molecular mechanisms by which sanggenon C mediated the induction of cell death in colorectal cancer cells (CRC). Treatment of colorectal cancer cells (LoVo, HT-29 and SW480) with sanggenon C (0, 5, 10, 20, 40 and 80 µM) resulted in inhibited proliferation of colon cancer cells. In addition, Sanggenon C (10, 20, 40 µM) induces apoptosis of HT-29 colon cancer cells as well as the increased ROS generation. Furthermore, treatment with sanggenon C increased the level of intracellular Ca2+ and ATP, while inhibited the nitric oxide production via inhibiting inducible nitric oxide synthase expression. This resulted in the activation of mitochondrial apoptosis pathway as evidenced by the decrease in Bcl-2 protein expression. Consistently, the anti-growth and pro-apoptosis effects of sanggenon C on xenograft colon tumor were further confirmed in vivo. Collectively, our results demonstrated sanggenon C induced apoptosis of colon cancer cells by increased reactive oxygen species generation and decreased nitric oxide production, which is associated with inhibition of inducible nitric oxide synthase expression and activation of mitochondrial apoptosis pathway.
- Subjects :
- 0301 basic medicine
Cancer Research
Programmed cell death
Cell
sanggenon C
Nitric Oxide Synthase Type II
Biology
Nitric Oxide
Nitric oxide
03 medical and health sciences
chemistry.chemical_compound
Mice
0302 clinical medicine
medicine
Animals
Humans
Benzofurans
reactive oxygen species
Oncogene
apoptosis
General Medicine
Articles
Cell cycle
Molecular medicine
Molecular biology
Xenograft Model Antitumor Assays
Mitochondria
Nitric oxide synthase
Gene Expression Regulation, Neoplastic
030104 developmental biology
medicine.anatomical_structure
Oncology
chemistry
Apoptosis
Chromones
030220 oncology & carcinogenesis
Colonic Neoplasms
biology.protein
colon cancer cells
HT29 Cells
Subjects
Details
- Language :
- English
- ISSN :
- 17912431 and 1021335X
- Volume :
- 38
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Oncology Reports
- Accession number :
- edsair.doi.dedup.....f4a7a14353f2cd27f6056d425ebda7f3