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In vivo diagnosis with purified tropomyosin in mite and shellfish allergic patients
- Source :
- Annals of Allergy, Asthma & Immunology. 116:538-543
- Publication Year :
- 2016
- Publisher :
- Elsevier BV, 2016.
-
Abstract
- Background Tropomyosin is the most studied shellfish allergen and has been involved in cross-reactivity among different invertebrates (crustacean, mollusks, mites, insects, and nematodes). Objective To determine the relevance of tropomyosin in mite- and shellfish-sensitized patients using tropomyosin skin testing. Methods Patients were divided into 3 groups: group M included mite allergic patients (ie, individuals with respiratory symptoms and a positive result on skin prick testing [SPT] to house dust mites), group S included shellfish allergic patients (ie, individuals who reported symptoms with shellfish), and group MS included mite- and shellfish allergic patients (ie, individuals who simultaneously fulfilled the inclusion criteria for groups M and S). Tropomyosin was purified from shrimp, characterized, and used in SPT for diagnosis in the patient population. Results Eight hundred fifty patients were included in the study: 790 (92.9%) in group M, 21 (2.5%) in group S, and 39 (4.6%) in group MS. Tropomyosin was purified from shrimp with a purity higher than 95%. Forty-two individuals tested positive to tropomyosin: the prevalence was 2.7% in group M, 28.6% in group S, and 38.5% in patients of group MS. Twenty-one (50%) of the tropomyosin-positive individuals had symptoms with shellfish, and 3 (14.3%) reported anaphylaxis. Conclusion The prevalence of tropomyosin was low in mite-sensitized patients (2.7 %) and high in shellfish allergic patients (28.6%). The higher prevalence of tropomyosin was found in patients sensitized to both mite and shellfish (38.5%). The selection of tropomyosin-sensitized patients by SPT might help in the choice of appropriate treatments or management for these patients.
- Subjects :
- Adult
Male
0301 basic medicine
Pulmonary and Respiratory Medicine
medicine.medical_specialty
animal structures
Adolescent
Immunology
Tropomyosin
macromolecular substances
medicine.disease_cause
Gastroenterology
Young Adult
03 medical and health sciences
0302 clinical medicine
Allergen
In vivo
Internal medicine
Hypersensitivity
Prevalence
medicine
Mite
Animals
Humans
Immunology and Allergy
In patient
Shellfish
Aged
Skin Tests
biology
business.industry
Pyroglyphidae
Allergens
Middle Aged
biology.organism_classification
medicine.disease
Shrimp
030104 developmental biology
030228 respiratory system
Spain
Female
business
Anaphylaxis
Subjects
Details
- ISSN :
- 10811206
- Volume :
- 116
- Database :
- OpenAIRE
- Journal :
- Annals of Allergy, Asthma & Immunology
- Accession number :
- edsair.doi.dedup.....f4a86c34cd53ae3ae81a2da66e5862bf