Back to Search
Start Over
A novel peptide with potent and broad-spectrum antiviral activities against multiple respiratory viruses
- Source :
- Scientific Reports
- Publication Year :
- 2016
- Publisher :
- Nature Publishing Group, 2016.
-
Abstract
- A safe, potent and broad-spectrum antiviral is urgently needed to combat emerging respiratory viruses. In light of the broad antiviral activity of β-defensins, we tested the antiviral activity of 11 peptides derived from mouse β-defensin-4 and found that a short peptide, P9, exhibited potent and broad-spectrum antiviral effects against multiple respiratory viruses in vitro and in vivo, including influenza A virus H1N1, H3N2, H5N1, H7N7, H7N9, SARS-CoV and MERS-CoV. The antiviral activity of P9 was attributed to its high-affinity binding to viral glycoproteins, as well as the abundance of basic amino acids in its composition. After binding viral particles through viral surface glycoproteins, P9 entered into cells together with the viruses via endocytosis and prevented endosomal acidification, which blocked membrane fusion and subsequent viral RNA release. This study has paved the avenue for developing new prophylactic and therapeutic agents with broad-spectrum antiviral activities.
- Subjects :
- 0301 basic medicine
beta-Defensins
viruses
Peptide
Endosomes
Microbial Sensitivity Tests
Biology
medicine.disease_cause
Endocytosis
Antiviral Agents
Article
03 medical and health sciences
Inhibitory Concentration 50
Mice
Orthomyxoviridae Infections
Viral Envelope Proteins
In vivo
Influenza A virus
medicine
Animals
chemistry.chemical_classification
Multidisciplinary
030102 biochemistry & molecular biology
Lipid bilayer fusion
virus diseases
Viral Load
Virology
In vitro
Peptide Fragments
030104 developmental biology
chemistry
Severe acute respiratory syndrome-related coronavirus
Middle East Respiratory Syndrome Coronavirus
Glycoprotein
Peptides
Viral load
Protein Binding
Subjects
Details
- Language :
- English
- ISSN :
- 20452322
- Database :
- OpenAIRE
- Journal :
- Scientific Reports
- Accession number :
- edsair.doi.dedup.....f4ea7b97474552b3b629470b6f0fd643
- Full Text :
- https://doi.org/10.1038/srep22008