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Changes of oxidative metabolism in the roots of wheat (Triticum aestivum L.) seedlings in response to elevated ammonium concentrations
- Source :
- Journal of Integrative Agriculture, Vol 20, Iss 5, Pp 1216-1228 (2021)
- Publication Year :
- 2021
- Publisher :
- Elsevier, 2021.
-
Abstract
- To elucidate the response of oxidative metabolism, triggered by elevated ammonium (NH4+) concentrations, on root growth of wheat seedlings, Yumai 49 (NH4+-tolerant) and Lumai 15 (NH4+-sensitive) cultivars were supplied with either 5.0 mmol Lā1 NH4+-N (EAC) or 5.0 mmol Lā1 NO3ā-N (CON) under hydroponic conditions. Root growth in both cultivars was significantly reduced under EAC, and the negative effect was greater in Lumai 15. EAC enhanced the activities of monodehydroascorbate reductase and dehydroascorbate reductase in the roots of both cultivars, while it decreased ascorbic acid (ASA) content and GDP-mannose pyrophosphorylase (GMPase) activity at the 12th day after treatment in Lumai 15 by 62.0 and 71.4%; and in Yumai 49 by 38.8 and 62.2%, respectively, indicating that the regeneration of ASA was increased, but the biosynthesis of ASA was reduced under EAC treatment. Moreover, EAC increased DHA/ASA, reactive oxygen species (ROS), and malondialdehyde contents, as well as antioxidant enzyme activities in the roots of both cultivars. Relatively greater increases in ROS and soluble sugar, and lower antioxidant enzyme activities in Lumai 15 indicate severe disruption of oxidative metabolism when compared to Yumai 49. Results reveal that the reduction of ASA biosynthesis via decreased GMPase activity under the EAC condition probably acts as a trigger for accumulated ROS and imbalanced redox status, resulting in root growth inhibition during wheat seedling growth stage. Yumai 49, being an NH4+ -tolerant cultivar, had the stronger capacity to protect itself from oxidative stress, which allowed it to retain a lower DHA to ASA ratio by maintaining a better redox homeostasis than could be maintained in the NH4+ -sensitive cultivar Lumai 15.
- Subjects :
- 0106 biological sciences
Antioxidant
medicine.medical_treatment
Agriculture (General)
Plant Science
Reductase
medicine.disease_cause
01 natural sciences
Biochemistry
S1-972
chemistry.chemical_compound
Food Animals
elevated ammonium concentrations
oxidative metabolism
wheat
medicine
Ammonium
Sugar
root morphology
chemistry.chemical_classification
Reactive oxygen species
redox homeostasis
Ecology
biology
food and beverages
04 agricultural and veterinary sciences
biology.organism_classification
Malondialdehyde
Horticulture
chemistry
Seedling
040103 agronomy & agriculture
0401 agriculture, forestry, and fisheries
Animal Science and Zoology
Agronomy and Crop Science
Oxidative stress
010606 plant biology & botany
Food Science
Subjects
Details
- Language :
- English
- ISSN :
- 20953119
- Volume :
- 20
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Journal of Integrative Agriculture
- Accession number :
- edsair.doi.dedup.....f4fa3d0fe9bd24c8225342dd40ee6ad9