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Alternative splicing and expression analysis of HSF1 in diapause pupal brains in the cotton bollworm, Helicoverpa armigera
- Source :
- Pest management science. 75(5)
- Publication Year :
- 2018
-
Abstract
- Background Diapause is the arrest of the development of insects and can be used for the development of effective agricultural pest management strategies. Heat shock protein 70 (Hsp70) is reported to be up-regulated during diapause to maintain survival in some insect species. However, its regulatory mechanism is unknown. Results Expression of hsp70 in Helicoverpa armigera was found to be up-regulated in diapause pupal brains. To elucidate the molecular regulatory mechanisms of hsp70, we focused our attention on its transcription factor, heat shock factor 1 (HSF1). Four alternative splicing variants of HSF1 from pupal brains of H. armigera were identified, and subcellular localization analysis indicated that these variants were exclusively expressed in the nucleus. Real-time PCR analysis showed that all of these variants were up-regulated in diapause pupal brains, and their expression patterns were consistent with that of hsp70. Finally, promoter activity assay and Western blotting detection demonstrated that hsp70 was activated and up-regulated by these variants. Conclusion Expression of hsp70 in H. armigera during diapause is regulated by multiple alternatively spliced isoforms of HSF1. The results of this study may provide important information for understanding the regulatory mechanisms of hsps during insect diapause. © 2018 Society of Chemical Industry.
- Subjects :
- 0106 biological sciences
media_common.quotation_subject
Intracellular Space
Insect
Diapause
Biology
Helicoverpa armigera
01 natural sciences
Gene expression
Animals
Amino Acid Sequence
HSF1
Promoter Regions, Genetic
Transcription factor
media_common
fungi
Alternative splicing
Pupa
Brain
General Medicine
biology.organism_classification
Cell biology
Lepidoptera
010602 entomology
Alternative Splicing
Protein Transport
Insect Science
Insect Proteins
Agronomy and Crop Science
Sequence Alignment
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15264998
- Volume :
- 75
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Pest management science
- Accession number :
- edsair.doi.dedup.....f5466183cd4fa44a0fb1ed9f196d5c5b