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Xiao Yao San against Corticosterone-Induced Stress Injury via Upregulating Glucocorticoid Receptor Reaction Element Transcriptional Activity
- Source :
- Evidence-Based Complementary and Alternative Medicine, Vol 2016 (2016), Evidence-based Complementary and Alternative Medicine : eCAM
- Publication Year :
- 2016
- Publisher :
- Hindawi Publishing Corporation, 2016.
-
Abstract
- Previous studies have revealed that uncontrollable stress can impair the synaptic plasticity and firing property of hippocampal neurons, which influenced various hippocampal-dependent tasks including memory, cognition, behavior, and mood. In this work, we had investigated the effects and mechanisms of the Chinese herbal medicine Xiao Yao San (XYS) against corticosterone-induced stress injury in primary hippocampal neurons (PHN) cells. We found that XYS and RU38486 could increase cell viabilities and decrease cell apoptosis by MTT, immunofluorescence, and flow cytometry assays. In addition, we observed that XYS notably inhibited the nuclear translocation of GR and upregulated the mRNA and protein expressions levels of Caveolin-1, GR, BDNF, TrkB, and FKBP4. However, XYS downregulated the FKBP51 expressions. Furthermore, the results of the electrophoretic mobility shift assay (EMSA) and double luciferase reporter gene detection indicated that FKBP4 promotes the transcriptional activity of GR reaction element (GRE) by binding with GR, and FKBP51 processed the opposite action. Thein vivoexperiment also proved the functions of XYS. These results suggested that XYS showed an efficient neuroprotection against corticosterone-induced stress injury in PHN cells by upregulating GRE transcriptional activity, which should be developed as a potential candidate for treating stress injury in the future.
- Subjects :
- 0301 basic medicine
Article Subject
Chemistry
lcsh:Other systems of medicine
Tropomyosin receptor kinase B
lcsh:RZ201-999
Neuroprotection
Cell biology
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
0302 clinical medicine
Glucocorticoid receptor
Complementary and alternative medicine
Downregulation and upregulation
Apoptosis
Corticosterone
Synaptic plasticity
Immunology
Electrophoretic mobility shift assay
030217 neurology & neurosurgery
Research Article
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- OpenAIRE
- Journal :
- Evidence-Based Complementary and Alternative Medicine
- Accession number :
- edsair.doi.dedup.....f55345734a00400f73992438cdc794d2
- Full Text :
- https://doi.org/10.1155/2016/5850739