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Hydrogen Sulfide Improves the Cold Stress Resistance through the CsARF5-CsDREB3 Module in Cucumber
- Source :
- International Journal of Molecular Sciences, International Journal of Molecular Sciences, Vol 22, Iss 13229, p 13229 (2021), International Journal of Molecular Sciences; Volume 22; Issue 24; Pages: 13229
- Publication Year :
- 2021
- Publisher :
- MDPI, 2021.
-
Abstract
- As an important gas signaling molecule, hydrogen sulfide (H2S) plays a crucial role in regulating cold tolerance. H2S cooperates with phytohormones such as abscisic acid, ethylene, and salicylic acid to regulate the plant stress response. However, the synergistic regulation of H2S and auxin in the plant response to cold stress has not been reported. This study showed that sodium hydrosulfide (NaHS, an H2S donor) treatment enhanced the cold stress tolerance of cucumber seedlings and increased the level of auxin. CsARF5, a cucumber auxin response factor (ARF) gene, was isolated, and its role in regulating H2S-mediated cold stress tolerance was described. Transgenic cucumber leaves overexpressing CsARF5 were obtained. Physiological analysis indicated that overexpression of CsARF5 enhanced the cold stress tolerance of cucumber and the regulation of the cold stress response by CsARF5 depends on H2S. In addition, molecular assays showed that CsARF5 modulated cold stress response by directly activating the expression of the dehydration-responsive element-binding (DREB)/C-repeat binding factor (CBF) gene CsDREB3, which was identified as a positive regulator of cold stress. Taken together, the above results suggest that CsARF5 plays an important role in H2S-mediated cold stress in cucumber. These results shed light on the molecular mechanism by which H2S regulates cold stress response by mediating auxin signaling; this will provide insights for further studies on the molecular mechanism by which H2S regulates cold stress. The aim of this study was to explore the molecular mechanism of H2S regulating cold tolerance of cucumber seedlings and provide a theoretical basis for the further study of cucumber cultivation and environmental adaptability technology in winter.
- Subjects :
- Cold tolerance
QH301-705.5
Transgene
Hydrogen sulfide
DREB
Regulator
hydrogen sulfide
Sodium hydrosulfide
ARF
auxin
cold stress
cucumber
module
resistance
Sulfides
Catalysis
Article
Inorganic Chemistry
chemistry.chemical_compound
Auxin
Gene Expression Regulation, Plant
Physical and Theoretical Chemistry
Biology (General)
Gene
Molecular Biology
QD1-999
Cold stress
Spectroscopy
Plant Proteins
chemistry.chemical_classification
Indoleacetic Acids
Chemistry
Cold-Shock Response
Gene Expression Profiling
Organic Chemistry
General Medicine
equipment and supplies
Plants, Genetically Modified
Cell biology
Computer Science Applications
DNA-Binding Proteins
Cucumis sativus
Subjects
Details
- Language :
- English
- ISSN :
- 14220067
- Volume :
- 22
- Issue :
- 24
- Database :
- OpenAIRE
- Journal :
- International Journal of Molecular Sciences
- Accession number :
- edsair.doi.dedup.....f572e111e681e935921c5fbb465185a0