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Fine genetic mapping of greenbug aphid-resistance gene Gb3 in Aegilops tauschii
- Source :
- Theoretical and Applied Genetics. 124:555-564
- Publication Year :
- 2011
- Publisher :
- Springer Science and Business Media LLC, 2011.
-
Abstract
- The greenbug, Schizaphis graminum (Rondani), is an important aphid pest of small grain crops especially wheat (Triticum aestivum L., 2n = 6x = 42, genomes AABBDD) in many parts of the world. The greenbug-resistance gene Gb3 originated from Aegilops tauschii Coss. (2n = 2x = 14, genome D(t)D(t)) has shown consistent and durable resistance against prevailing greenbug biotypes in wheat fields. We previously mapped Gb3 in a recombination-rich, telomeric bin of wheat chromosome arm 7DL. In this study, high-resolution genetic mapping was carried out using an F(2:3) segregating population derived from two Ae. tauschii accessions, the resistant PI 268210 (original donor of Gb3 in the hexaploid wheat germplasm line 'Largo') and susceptible AL8/78. Molecular markers were developed by exploring bin-mapped wheat RFLPs, SSRs, ESTs and the Ae. tauschii physical map (BAC contigs). Wheat EST and Ae. tauschii BAC end sequences located in the deletion bin 7DL3-0.82-1.00 were used to design STS (sequence tagged site) or CAPS (Cleaved Amplified Polymorphic Sequence) markers. Forty-five PCR-based markers were developed and mapped to the chromosomal region spanning the Gb3 locus. The greenbug-resistance gene Gb3 now was delimited in an interval of 1.1 cM by two molecular markers (HI067J6-R and HI009B3-R). This localized high-resolution genetic map with markers closely linked to Gb3 lays a solid foundation for map based cloning of Gb3 and marker-assisted selection of this gene in wheat breeding.
- Subjects :
- Genetic Markers
Germplasm
Chromosomes, Artificial, Bacterial
Population
Locus (genetics)
Breeding
Genes, Plant
Poaceae
Sequence-tagged site
Gene mapping
Cleaved amplified polymorphic sequence
Genetics
Animals
Aegilops tauschii
Amplified Fragment Length Polymorphism Analysis
education
Disease Resistance
Plant Diseases
Sequence Tagged Sites
Expressed Sequence Tags
education.field_of_study
biology
Chromosome Mapping
food and beverages
General Medicine
biology.organism_classification
Aphids
Chromosomal region
Agronomy and Crop Science
Biotechnology
Subjects
Details
- ISSN :
- 14322242 and 00405752
- Volume :
- 124
- Database :
- OpenAIRE
- Journal :
- Theoretical and Applied Genetics
- Accession number :
- edsair.doi.dedup.....f5761b21963d02e7e39a8ed516e21ceb