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MAL regulates clathrin-mediated endocytosis at the apical surface of Madin–Darby canine kidney cells
- Source :
- The Journal of Cell Biology
- Publication Year :
- 2003
- Publisher :
- The Rockefeller University Press, 2003.
-
Abstract
- MAL is an integral protein component of the machinery for apical transport in epithelial Madin–Darby canine kidney (MDCK) cells. To maintain its distribution, MAL cycles continuously between the plasma membrane and the Golgi complex. The clathrin-mediated route for apical internalization is known to differ from that at the basolateral surface. Herein, we report that MAL depends on the clathrin pathway for apical internalization. Apically internalized polymeric Ig receptor (pIgR), which uses clathrin for endocytosis, colocalized with internalized MAL in the same apical vesicles. Time-lapse confocal microscopic analysis revealed cotransport of pIgR and MAL in the same endocytic structures. Immunoelectron microscopic analysis evidenced colabeling of MAL with apically labeled pIgR in pits and clathrin-coated vesicles. Apical internalization of pIgR was abrogated in cells with reduced levels of MAL, whereas this did not occur either with its basolateral entry or the apical internalization of glycosylphosphatidylinositol-anchored proteins, which does not involve clathrin. Therefore, MAL is critical for efficient clathrin-mediated endocytosis at the apical surface in MDCK cells.
- Subjects :
- Glycosylphosphatidylinositols
media_common.quotation_subject
Endocytic cycle
education
Biology
Endocytosis
Kidney
Clathrin
Article
symbols.namesake
Mice
Dogs
apical endocytosis
protein machinery
polarized transport
epithelial cells
lipid rafts
parasitic diseases
Receptors, Transferrin
Animals
Internalization
media_common
Vesicle
Receptors, Polymeric Immunoglobulin
Membrane Proteins
Cell Biology
Receptor-mediated endocytosis
Golgi apparatus
Oligonucleotides, Antisense
Cell biology
Membrane protein
symbols
biology.protein
lipids (amino acids, peptides, and proteins)
Subjects
Details
- Language :
- English
- ISSN :
- 15408140 and 00219525
- Volume :
- 163
- Issue :
- 1
- Database :
- OpenAIRE
- Journal :
- The Journal of Cell Biology
- Accession number :
- edsair.doi.dedup.....f57f853fce46a77882b2ccc0c5e512fe