Back to Search
Start Over
Novel Approach for Simultaneous Analysis of Peptide Metabolites from Orally Administered Glycinin in Rat Bloodstream by Coumarin-Tagged MALDIāMS
- Source :
- Journal of Agricultural and Food Chemistry. 69:14840-14848
- Publication Year :
- 2021
- Publisher :
- American Chemical Society (ACS), 2021.
-
Abstract
- The lack of an appropriate analytical approach characterizing metabolites from dietary proteins may prevent further studies that could clarify their health benefits. In this study, we attempted to establish a novel analytical assay of peptide metabolites from glycinin using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), in combination with the amine derivatization technique with coumarin (Cou). Cou (30 mmol/L) derivatization of peptides under rapid (30 min) and mild (25 °C, pH 8.5) conditions caused higher MS detection of the peptides as compared to nonderivatized peptides. In addition, an MS shift of the target by Cou derivatization (+202.0 m/z) can help to easily discriminate peptide metabolites in glycinin-administered blood, by comparing the MALDI-MS spectra of Cou-derivatized plasma with those of preadministered blood. After the oral administration of glycinin (100 mg/kg) to Sprague-Dawley rats, 15 di- to tetrapeptides were successfully characterized as glycinin-derived metabolites, demonstrating that the proposed Cou-tagged MALDI-MS is an appropriate characterization technique for peptide metabolites.
- Subjects :
- chemistry.chemical_classification
Maldi ms
Chromatography
Metabolite
Globulins
Peptide
General Chemistry
Coumarin
Mass spectrometry
Rats
Rats, Sprague-Dawley
chemistry.chemical_compound
chemistry
Coumarins
Oral administration
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Soybean Proteins
Animals
Amine gas treating
Peptides
General Agricultural and Biological Sciences
Derivatization
Subjects
Details
- ISSN :
- 15205118 and 00218561
- Volume :
- 69
- Database :
- OpenAIRE
- Journal :
- Journal of Agricultural and Food Chemistry
- Accession number :
- edsair.doi.dedup.....f5842dcc9a673cef04e4d03bff2a9d73