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Novel Approach for Simultaneous Analysis of Peptide Metabolites from Orally Administered Glycinin in Rat Bloodstream by Coumarin-Tagged MALDIā€“MS

Authors :
Satoshi Nagaoka
Risa Katagihara
Mitsuru Tanaka
Marika Hashimoto
Xiaojing Sheng
Toshiro Matsui
Source :
Journal of Agricultural and Food Chemistry. 69:14840-14848
Publication Year :
2021
Publisher :
American Chemical Society (ACS), 2021.

Abstract

The lack of an appropriate analytical approach characterizing metabolites from dietary proteins may prevent further studies that could clarify their health benefits. In this study, we attempted to establish a novel analytical assay of peptide metabolites from glycinin using matrix-assisted laser desorption/ionization-mass spectrometry (MALDI-MS), in combination with the amine derivatization technique with coumarin (Cou). Cou (30 mmol/L) derivatization of peptides under rapid (30 min) and mild (25 °C, pH 8.5) conditions caused higher MS detection of the peptides as compared to nonderivatized peptides. In addition, an MS shift of the target by Cou derivatization (+202.0 m/z) can help to easily discriminate peptide metabolites in glycinin-administered blood, by comparing the MALDI-MS spectra of Cou-derivatized plasma with those of preadministered blood. After the oral administration of glycinin (100 mg/kg) to Sprague-Dawley rats, 15 di- to tetrapeptides were successfully characterized as glycinin-derived metabolites, demonstrating that the proposed Cou-tagged MALDI-MS is an appropriate characterization technique for peptide metabolites.

Details

ISSN :
15205118 and 00218561
Volume :
69
Database :
OpenAIRE
Journal :
Journal of Agricultural and Food Chemistry
Accession number :
edsair.doi.dedup.....f5842dcc9a673cef04e4d03bff2a9d73