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Measurement of nanoscale DNA translocation by uracil DNA glycosylase in human cells
- Source :
- Nucleic Acids Research
- Publication Year :
- 2017
- Publisher :
- Oxford University Press (OUP), 2017.
-
Abstract
- DNA ‘sliding’ by human repair enzymes is considered to be important for DNA damage detection. Here, we transfected uracil-containing DNA duplexes into human cells and measured the probability that nuclear human uracil DNA glycosylase (hUNG2) excised two uracil lesions spaced 10–80 bp apart in a single encounter without escaping the micro-volume containing the target sites. The two-site transfer probabilities were 100% and 54% at a 10 and 40 bp spacing, but dropped to only 10% at 80 bp. Enzyme trapping experiments suggested that site transfers over 40 bp followed a DNA ‘hopping’ pathway in human cells, indicating that authentic sliding does not occur even over this short distance. The transfer probabilities were much greater than observed in aqueous buffers, but similar to in vitro measurements in the presence of polymer crowding agents. The findings reveal a new role for the crowded nuclear environment in facilitating DNA damage detection.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
Nucleic Acid Enzymes
DNA damage
Uracil
DNA
Transfection
Biology
Cell Line
DNA Glycosylases
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Enzyme
chemistry
DNA glycosylase
Uracil-DNA glycosylase
Genetics
Biophysics
Humans
A-DNA
Subjects
Details
- ISSN :
- 13624962 and 03051048
- Volume :
- 45
- Database :
- OpenAIRE
- Journal :
- Nucleic Acids Research
- Accession number :
- edsair.doi.dedup.....f59a30fe51cc39a755fa97d1d2dea8c5