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KLF4 Inhibits the Differentiation of Goat Intramuscular Preadipocytes Through Targeting C/EBPβ Directly
- Source :
- Frontiers in Genetics, Vol 12 (2021), Frontiers in Genetics
- Publication Year :
- 2021
- Publisher :
- Frontiers Media S.A., 2021.
-
Abstract
- Intramuscular fat (IMF) deposition is a complicated process, and most of the underlying regulators of this biological process are unknown. Here, we cloned the intact CDS of KLF4 gene, investigated the role of KLF4 by gaining or losing function in vitro and further explored the pathways of KLF4 regulating differentiation of intramuscular preadipocytes in goat. Our results show that goat KLF4 gene consists of 1,536 bp encoding a protein of 486 amino acids. The expression of KLF4 is higher in the lung while lower in the heart and muscle in goat. Knockdown of KLF4 mediated by siRNA technique significantly promotes intramuscular preadipocyte lipid accumulation and upregulates mRNA expression of adipogenic related genes including C/EBPα, C/EBPβ, and PPARγ in vivo cultured cells. Consistently, overexpression of KLF4 inhibits intramuscular adipocyte lipid accumulation and significantly downregulation gene expression of C/EBPβ, PPARγ, aP2, and Pref-1. Further, we found that other members of KLFs were upregulated or downregulated after interference or overexpression of KLF4, including KLF2 and KLF5–7. We also found that C/EBPβ was a potential target of KLF4, because it had an opposite expression pattern with KLF4 during the differentiation of intramuscular preadipocytes and had putative binding sites of KLF4. The dual-luciferase reporter assay indicated that overexpression of KLF4 inhibited the transcriptional activity of C/EBPβ. These results demonstrate that KLF4 inhibits the differentiation of intramuscular preadipocytes in goat by targeting C/EBPβ.
- Subjects :
- 0301 basic medicine
QH426-470
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
stomatognathic system
Adipocyte
Gene expression
Genetics
Genetics (clinical)
Original Research
Gene knockdown
Reporter gene
intramuscular preadipocytes
Chemistry
fungi
goat
differentiation
KLF4
Cell biology
030104 developmental biology
Adipogenesis
030220 oncology & carcinogenesis
KLF2
embryonic structures
C/EBPβ
Molecular Medicine
Intramuscular fat
sense organs
biological phenomena, cell phenomena, and immunity
Subjects
Details
- Language :
- English
- ISSN :
- 16648021
- Volume :
- 12
- Database :
- OpenAIRE
- Journal :
- Frontiers in Genetics
- Accession number :
- edsair.doi.dedup.....f5f260f965347f1389e896a4587206dd
- Full Text :
- https://doi.org/10.3389/fgene.2021.663759/full