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Magnesium Ions But Not ATP Inhibit Cyclic ADP-Ribose-Induced Calcium Release
- Source :
- Biochemical and Biophysical Research Communications. 206:786-791
- Publication Year :
- 1995
- Publisher :
- Elsevier BV, 1995.
-
Abstract
- The pharmacology of the cyclic ADP-ribose (cADPR)-dependent Ca2+ release mechanism is very similar to that of the ryanodine receptor (RyR). Here we showed that MgCl2, a known inhibitor of RyR, blocked cADPR-induced Ca+2 release in sea urchin egg homogenates with a half maximal concentration of about 2.5 mM. The effect was specific since up to 10 mM Mg+2 had no effect on the Ca+2 release induced by inositol trisphosphate. K2ATP, another known modulator of RyR, at up to 10 mM did not affect the half-maximal concentration of cADPR, which remained at about 96 nM. These results indicate cADPR is a specific Ca+2 release activator and not merely an adenine nucleotide acting on the ATP-site. The inhibitory effects of Mg+2 further demonstrate the similarity between RyR and the cADPR-dependent Ca+2 release system.
- Subjects :
- inorganic chemicals
Magnesium Chloride
Biophysics
chemistry.chemical_element
Calcium
Inhibitory postsynaptic potential
Biochemistry
Cyclic ADP-ribose
chemistry.chemical_compound
Adenosine Triphosphate
Adenine nucleotide
Animals
Molecular Biology
Magnesium ion
Adenosine Diphosphate Ribose
Cyclic ADP-Ribose
Chemistry
Ryanodine receptor
Activator (genetics)
Inositol trisphosphate
Cell Biology
Kinetics
Sea Urchins
Oocytes
Female
Subjects
Details
- ISSN :
- 0006291X
- Volume :
- 206
- Database :
- OpenAIRE
- Journal :
- Biochemical and Biophysical Research Communications
- Accession number :
- edsair.doi.dedup.....f6191583d54e13a9b26896e8dc2a615b