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Multicolorimetric ELISA biosensors on a paper/polymer hybrid analytical device for visual point-of-care detection of infection diseases
- Source :
- Analytical and Bioanalytical Chemistry
- Publication Year :
- 2021
- Publisher :
- Springer Science and Business Media LLC, 2021.
-
Abstract
- Graphical abstract Enzyme-linked immunosorbent assay (ELISA) is widely used for the detection of disease biomarkers. However, it utilizes time-consuming procedures and expensive instruments, making it infeasible for point-of-care (POC) analysis especially in resource-limited settings. In this work, a multicolorimetric ELISA biosensor integrated on a paper/polymer hybrid microfluidic device was developed for rapid visual detection of disease biomarkers at point of care, without using costly equipment. This multicolormetric ELISA platform was built on multiple distinct color variants resulted from the catalytic oxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) and the etching of gold nanorods (AuNRs). The vivid color changes could be easily distinguished by the naked eye, and their red mean values allowed quantitative biomarker detection, without using any sophisticated instruments. When this multicolorimetric ELISA was integrated on a paper/polymer hybrid analytical device, it not only provided integrated processing and high portability but also enabled fast assays in about 50 min due to the unique advantages of paper/polymer hybrid devices. The limit of detection of 9.1 ng/μL of the hepatitis C virus core antigen, a biomarker for hepatitis C, was achieved using this multicolorimetric ELISA platform. This multicolor ELISA analytical device provides a new versatile, user-friendly, affordable, and portable immunosensing platform with high potential for on-site detections of various viruses, proteins, and biomarkers for low-resource settings such as at home, public venues, rural areas, and developing nations. Supplementary Information The online version contains supplementary material available at 10.1007/s00216-021-03359-8.
- Subjects :
- Paper
Polymers
Computer science
Point-of-Care Systems
Point-of-care detection
Enzyme-Linked Immunosorbent Assay
Biosensing Techniques
02 engineering and technology
Communicable Diseases
Sensitivity and Specificity
Multicolorimetric ELISA
01 natural sciences
Biochemistry
Analytical Chemistry
Humans
Disease biomarker
Integrated processing
Paper/polymer hybrid microfluidic device
Horseradish Peroxidase
High potential
Point of care
Detection limit
Quantitative biomarker detection
Infectious disease
business.industry
010401 analytical chemistry
Hydrogen Peroxide
021001 nanoscience & nanotechnology
0104 chemical sciences
Visual detection
Embedded system
Colorimetry
Spectrophotometry, Ultraviolet
Hepatitis C virus core Antigen
0210 nano-technology
business
Biosensor
Biomarkers
Research Paper
Subjects
Details
- ISSN :
- 16182650 and 16182642
- Volume :
- 413
- Database :
- OpenAIRE
- Journal :
- Analytical and Bioanalytical Chemistry
- Accession number :
- edsair.doi.dedup.....f6592d7f0aa7980cd9cc624dde916d4a