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Bacterial RNA Biology on a Genome Scale

Bacterial RNA Biology on a Genome Scale

Authors :
Jörg Vogel
Stanislaw A. Gorski
Jens Hör
HIRI, Helmholtz-Institut für RNA-basierte Infektionsforschung, Josef-Schneider-Strasse 2, 97080 Würzburg, Germany.
Source :
Molecular Cell. 70:785-799
Publication Year :
2018
Publisher :
Elsevier BV, 2018.

Abstract

Bacteria are an exceedingly diverse group of organisms whose molecular exploration is experiencing a renaissance. While the classical view of bacterial gene expression was relatively simple, the emerging view is more complex, encompassing extensive post-transcriptional control involving riboswitches, RNA thermometers, and regulatory small RNAs (sRNAs) associated with the RNA-binding proteins CsrA, Hfq, and ProQ, as well as CRISPR/Cas systems that are programmed by RNAs. Moreover, increasing interest in members of the human microbiota and environmental microbial communities has highlighted the importance of understudied bacterial species with largely unknown transcriptome structures and RNA-based control mechanisms. Collectively, this creates a need for global RNA biology approaches that can rapidly and comprehensively analyze the RNA composition of a bacterium of interest. We review such approaches with a focus on RNA-seq as a versatile tool to investigate the different layers of gene expression in which RNA is made, processed, regulated, modified, translated, and turned over.

Details

ISSN :
10972765
Volume :
70
Database :
OpenAIRE
Journal :
Molecular Cell
Accession number :
edsair.doi.dedup.....f67b1572d55fa5e9cd32d8391c7e6414
Full Text :
https://doi.org/10.1016/j.molcel.2017.12.023