Back to Search
Start Over
Bacterial RNA Biology on a Genome Scale
Bacterial RNA Biology on a Genome Scale
- Source :
- Molecular Cell. 70:785-799
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Bacteria are an exceedingly diverse group of organisms whose molecular exploration is experiencing a renaissance. While the classical view of bacterial gene expression was relatively simple, the emerging view is more complex, encompassing extensive post-transcriptional control involving riboswitches, RNA thermometers, and regulatory small RNAs (sRNAs) associated with the RNA-binding proteins CsrA, Hfq, and ProQ, as well as CRISPR/Cas systems that are programmed by RNAs. Moreover, increasing interest in members of the human microbiota and environmental microbial communities has highlighted the importance of understudied bacterial species with largely unknown transcriptome structures and RNA-based control mechanisms. Collectively, this creates a need for global RNA biology approaches that can rapidly and comprehensively analyze the RNA composition of a bacterium of interest. We review such approaches with a focus on RNA-seq as a versatile tool to investigate the different layers of gene expression in which RNA is made, processed, regulated, modified, translated, and turned over.
- Subjects :
- 0301 basic medicine
Riboswitch
Small RNA
RNA Stability
030106 microbiology
RNA-binding protein
Computational biology
Biology
Structure-Activity Relationship
03 medical and health sciences
Bacterial Proteins
Transcription (biology)
CRISPR
RNA Processing, Post-Transcriptional
Molecular Biology
Post-transcriptional regulation
Bacteria
Sequence Analysis, RNA
Gene Expression Profiling
RNA
Gene Expression Regulation, Bacterial
Cell Biology
Non-coding RNA
RNA, Bacterial
030104 developmental biology
Protein Biosynthesis
Nucleic Acid Conformation
Transcriptome
Genome, Bacterial
Subjects
Details
- ISSN :
- 10972765
- Volume :
- 70
- Database :
- OpenAIRE
- Journal :
- Molecular Cell
- Accession number :
- edsair.doi.dedup.....f67b1572d55fa5e9cd32d8391c7e6414
- Full Text :
- https://doi.org/10.1016/j.molcel.2017.12.023