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Impact of salt exposure on N-acetylgalactosamine-4-sulfatase (arylsulfatase B) activity, glycosaminoglycans, kininogen, and bradykinin
- Source :
- Glycoconjugate Journal. 30:667-676
- Publication Year :
- 2013
- Publisher :
- Springer Science and Business Media LLC, 2013.
-
Abstract
- N -acetylgalactosamine-4-sulfatase (Arylsulfatase B; ARSB) is the enzyme that removes sulfate groups from the N-acetylgalactosamine-4-sulfate residue at the non-reducing end of chondroitin-4-sulfate (C4S) and dermatan sulfate (DS). Previous studies demonstrated reduction in cell-bound high molecular weight kininogen in normal rat kidney (NRK) epithelial cells when chondroitin-4-sulfate content was reduced following overexpression of ARSB activity, and chondroitinase ABC produced similar decline in cell-bound kininogen. Reduction in the cell-bound kininogen was associated with increase in secreted bradykinin. In this report, we extend the in vitro findings to in vivo models, and present findings in Dahl salt-sensitive (SS) rats exposed to high (SSH) and low salt (SSL) diets. In the renal tissue of the SSH rats, ARSB activity was significantly less than in the SSL rats, and chondroitin-4-sulfate and total sulfated glycosaminoglycan content were significantly greater. Disaccharide analysis confirmed marked increase in C4S disaccharides in the renal tissue of the SSH rats. In contrast, unsulfated, hyaluronan-derived disaccharides were increased in the rats on the low salt diet. In the SSH rats, with lower ARSB activity and higher C4S levels, cell-bound, high-molecular weight kininogen was greater and urinary bradykinin was lower. ARSB activity in renal tissue and NRK cells declined when exogenous chloride concentration was increased in vitro. The impact of high chloride exposure in vivo on ARSB, chondroitin-4-sulfation, and C4S-kininogen binding provides a mechanism that links dietary salt intake with bradykinin secretion and may be a factor in blood pressure regulation.
- Subjects :
- Arylsulfatase B
medicine.medical_specialty
N-Acetylgalactosamine-4-Sulfatase
High-molecular-weight kininogen
Bradykinin
Disaccharides
Kidney
Biochemistry
Article
Dermatan sulfate
Cell Line
chemistry.chemical_compound
Chlorides
Internal medicine
medicine
Animals
Hyaluronic Acid
Molecular Biology
Glycosaminoglycans
Kininogen
Rats, Inbred Dahl
Kininogens
Sulfates
Sulfatase
Chondroitin Sulfates
Epithelial Cells
Sodium, Dietary
Cell Biology
Diet, Sodium-Restricted
Rats
Endocrinology
medicine.anatomical_structure
chemistry
Subjects
Details
- ISSN :
- 15734986 and 02820080
- Volume :
- 30
- Database :
- OpenAIRE
- Journal :
- Glycoconjugate Journal
- Accession number :
- edsair.doi.dedup.....f6bbed67d9269e0a17464bbcb00ed07e
- Full Text :
- https://doi.org/10.1007/s10719-013-9468-8