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Validation of a new method by nano-liquid chromatography on chip tandem mass spectrometry for combined quantitation of C3f and the V65 vitronectin fragment as biomarkers of diagnosis and severity of osteoarthritis
- Source :
- Talanta. 169
- Publication Year :
- 2017
-
Abstract
- Microfluidic liquid chromatography coupled to a nanoelectrospray source ion trap mass spectrometry was used for the absolute and simultaneous quantitation of C3f and the V65 vitronectin fragment in serum. The method was first carefully optimized and then validated in serum biological matrix. Stable isotopes for the two biomarkers of interest were used as stable isotope labeled peptide standards. A weighted 1/x2 quadratic regression for C3f and a weighted 1/x quadratic regression for the V65 vitronectin peptide were selected for calibration curves. Trueness (with a relative bias
- Subjects :
- Calibration curve
Biomedical Engineering
Peptide
Osteoarthritis
Mass spectrometry
Bioinformatics
Tandem mass spectrometry
01 natural sciences
Severity of Illness Index
Analytical Chemistry
Matrix (chemical analysis)
Cohort Studies
03 medical and health sciences
0302 clinical medicine
Rheumatology
Tandem Mass Spectrometry
medicine
Humans
Nanotechnology
Orthopedics and Sports Medicine
Vitronectin
030203 arthritis & rheumatology
chemistry.chemical_classification
Chromatography
biology
Fragment (computer graphics)
Chemistry
010401 analytical chemistry
Reproducibility of Results
Repeatability
medicine.disease
0104 chemical sciences
Complement C3b
biology.protein
Biomarkers
Chromatography, Liquid
Subjects
Details
- ISSN :
- 18733573
- Volume :
- 169
- Database :
- OpenAIRE
- Journal :
- Talanta
- Accession number :
- edsair.doi.dedup.....f6ced75d97e7a8b36ad37abb82d9e696