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Rhinovirus detection using different PCR-based strategies
- Source :
- Brazilian Journal of Microbiology, Brazilian Journal of Microbiology, Vol 43, Iss 2, Pp 739-743 (2012), Brazilian Journal of Microbiology, Volume: 43, Issue: 2, Pages: 739-743, Published: JUN 2012, Brazilian Journal of Microbiology v.43 n.2 2012, Sociedade Brasileira de Microbiologia (SBM), instacron:SBM
- Publication Year :
- 2012
- Publisher :
- FapUNIFESP (SciELO), 2012.
-
Abstract
- Human rhinoviruses (HRVs) are the major cause of the common cold. HRVs were recently reclassified into the Enterovirus genus (HEV) in the Picornaviridae family. HRVs and other members of the HEV genus share many common features, including sense RNA genomes and partial nucleotide sequence identity. The aim of this study was to evaluate different HRV detection strategies. Samples from adults with acute respiratory infection (n = 291) who were treated in Sao Paulo Hospital (2001-2003) were tested using three assays. The first assay detected picornaviruses by RT-PCR and hybridization, the second detected rhinoviruses using RT-PCR/sequencing, and the third differentiated HRV from HEV using duplex semi-nested-RT-PCR. Analysis of the results obtained from the first two strategies revealed 83% concordance. Discordant samples were then evaluated by the third protocol, and 82% were negative. The picornavirus detection protocol was more sensitive but less specific than the rhinovirus detection protocols. The semi-nested protocol utilized in the present study was less sensitive and was not useful in differentiating HRV from HEV. Sequencing assays examining different genes would address the best strategy of confirming rhinovirus and enterovirus infections.
- Subjects :
- Rhinovirus
Picornavirus
viruses
lcsh:QR1-502
Picornaviridae
Biology
medicine.disease_cause
Microbiology
lcsh:Microbiology
stomatognathic system
medicine
Gene
Enterovirus
Nucleic acid sequence
virus diseases
Respiratory infection
Common cold
Genetics and Molecular Microbiology
medicine.disease
biology.organism_classification
Virology
Detection
Differentiation
Research Paper
Subjects
Details
- ISSN :
- 15178382
- Volume :
- 43
- Database :
- OpenAIRE
- Journal :
- Brazilian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....f6f67f786c1ff6fb5adfec3b7acd7daf
- Full Text :
- https://doi.org/10.1590/s1517-83822012000200038