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Nodulation and nitrogen fixation by Mimosa spp. in the Cerrado and Caatinga biomes of Brazil
- Source :
- New Phytologist, New Phytologist, Wiley, 2010, 186 (4), pp.934-946. ⟨10.1111/j.1469-8137.2010.03267.x⟩, Digital.CSIC. Repositorio Institucional del CSIC, instname
- Publication Year :
- 2010
-
Abstract
- •An extensive survey of nodulation in the legume genus Mimosa was undertaken in two major biomes in Brazil, the Cerrado and the Caatinga, in both of which there are high degrees of endemicity of the genus.•Nodules were collected from 67 of the 70 Mimosa spp. found. Thirteen of the species were newly reported as nodulating. Nodules were examined by light and electron microscopy, and all except for M. gatesiae had a structure typical of effective Mimosa nodules. The endosymbiotic bacteria in nodules from all of the Mimosa spp. were identified as Burkholderia via immunolabelling with an antibody against Burkholderia phymatum STM815.•Twenty of the 23 Mimosa nodules tested were shown to contain nitrogenase by immunolabelling with an antibody to the nitrogenase Fe- (nifH) protein, and using the δ15N (15N natural abundance) technique, contributions by biological N2 fixation of up to 60% of total plant N were calculated for Caatinga Mimosa spp.•It is concluded that nodulation in Mimosa is a generic character, and that the preferred symbionts of Brazilian species are Burkholderia. This is the first study to demonstrate N2 fixation by beta-rhizobial symbioses in the field.<br />This work was funded by the Natural Environment Research Council, UK (NE/B505038/1). The collection and transfer of nodules were authorized by permit no. 007/2005 from the Ministério do Meio Ambiente, Brazil (IBAMA).
- Subjects :
- 0106 biological sciences
food.ingredient
Root nodule
Mimosa
Burkholderia
Physiology
Blotting, Western
Cupriavidus taiwanensis
Plant Science
01 natural sciences
Plant Root Nodulation
campo rupestre
03 medical and health sciences
food
Symbiosis
Nitrogen Fixation
Nitrogenase
Botany
Ecosystem
030304 developmental biology
0303 health sciences
biology
Geography
Nitrogen Isotopes
Acetylene
15N
Pantanal
15 N natural abundance
15. Life on land
Paraburkholderia
nitrogenase
biology.organism_classification
[SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology
Nitrogen fixation
Beta-rhizobia
beta-rhizobia
Campo rupestre
Oxidoreductases
Root Nodules, Plant
Oxidation-Reduction
Brazil
010606 plant biology & botany
Burkholderia phymatum
Rhizobium
Subjects
Details
- ISSN :
- 14698137 and 0028646X
- Volume :
- 186
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- The New phytologistReferences
- Accession number :
- edsair.doi.dedup.....f6faf062780d4eb15aa64f98dbd5461f