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Thrombin-Induced Increase in Endothelial Permeability Is Associated With Changes in Cell-to-Cell Junction Organization
- Source :
- Scopus-Elsevier
- Publication Year :
- 1996
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1996.
-
Abstract
- Abstract Thrombin increases endothelial permeability in a rapid and reversible way. This effect requires the catalytic activity of the enzyme and thrombin receptor engagement. Endothelial cell permeability is mostly regulated by intercellular junction organization. In the present study, we investigated whether opening of intercellular gaps after thrombin treatment could be related to changes in adherence-junction molecular organization. By immunofluorescence analysis, we found that thrombin stimulation of endothelial cells caused a marked alteration of the distribution of vascular endothelial (VE)-cadherin and of the associated catenins. These molecules, which are strictly localized at intercellular boundaries in confluent resting cells, were absent in the areas of intercellular retraction. Immunoprecipitation analysis indicated that thrombin disrupted the VE-cadherin/catenin complex. This effect was reversible and correlated with the increase in endothelial permeability. The use of a protein kinase C inhibitor (calphostin C) blocked both thrombin-induced permeability and disassembly of adherence-junction components. We propose that thrombin’s effect on endothelial cell junction organization is an important determinant in the increase in endothelial permeability induced by this agent.
- Subjects :
- Endothelium
Molecular Sequence Data
Biology
Thrombomodulin
Permeability
chemistry.chemical_compound
Thrombin
Thrombin receptor
medicine
Humans
Amino Acid Sequence
Phosphorylation
Cells, Cultured
Cadherins
Cell biology
Endothelial stem cell
Intercellular Junctions
Calphostin C
medicine.anatomical_structure
chemistry
Cell junction organization
Endothelium, Vascular
Catenin complex
Cardiology and Cardiovascular Medicine
medicine.drug
Subjects
Details
- ISSN :
- 15244636 and 10795642
- Volume :
- 16
- Database :
- OpenAIRE
- Journal :
- Arteriosclerosis, Thrombosis, and Vascular Biology
- Accession number :
- edsair.doi.dedup.....f7893e0ef91b919a73d8269823f1cbe1