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Dynamic acetylation profile during mammalian neurulation
- Source :
- Birth Defects Research
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Background Neural tube defects (NTDs) result from failure of neural tube closure during embryogenesis. These severe birth defects of the central nervous system include anencephaly and spina bifida, and affect 0.5–2 per 1,000 pregnancies worldwide in humans. It has been demonstrated that acetylation plays a pivotal role during neural tube closure, as animal models for defective histone acetyltransferase proteins display NTDs. Acetylation represents an important component of the complex network of posttranslational regulatory interactions, suggesting a possible fundamental role during primary neurulation events. This study aimed to assess protein acetylation contribution to early patterning of the central nervous system both in human and murine specimens. Methods We used both human and mouse (Cited2 −/−) samples to analyze the dynamic acetylation of proteins during embryo development through immunohistochemistry, western blot analysis and quantitative polymerase chain reaction. Results We report the dynamic profile of histone and protein acetylation status during neural tube closure. We also report a rescue effect in an animal model by chemical p53 inhibition. Conclusions Our data suggest that the p53‐acetylation equilibrium may play a role in primary neurulation in mammals.
- Subjects :
- p53
0301 basic medicine
Embryology
Health, Toxicology and Mutagenesis
Embryonic Development
030105 genetics & heredity
neural tube defect
Toxicology
Mice
03 medical and health sciences
acetylation profile
Western blot
Anencephaly
medicine
Animals
Humans
Neurulation
Spinal Dysraphism
Histone Acetyltransferases
Mammals
neurodevelopment
biology
medicine.diagnostic_test
Brief Report
Embryogenesis
Cited2
Neural tube
Acetylation
Histone acetyltransferase
medicine.disease
Cell biology
Repressor Proteins
Disease Models, Animal
030104 developmental biology
Histone
medicine.anatomical_structure
neural tube defects
Pediatrics, Perinatology and Child Health
Trans-Activators
biology.protein
Tumor Suppressor Protein p53
Transcription Factors
Developmental Biology
Subjects
Details
- ISSN :
- 24721727
- Volume :
- 112
- Database :
- OpenAIRE
- Journal :
- Birth Defects Research
- Accession number :
- edsair.doi.dedup.....f78af568ab12d29b27d14468fd9d04da