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Within-Host evolution of the Dutch high-prevalent pseudomonas aeruginosa clone ST406 during Chronic colonization of a patient with cystic fibrosis
- Source :
- PLoS ONE [E], 11(6). Public Library of Science, PLoS ONE, PLoS ONE, Vol 11, Iss 6, p e0158106 (2016), Van Mansfeld, R, De Been, M, Paganelli, F, Yang, L, Bonten, M & Willems, R 2016, ' Within-Host Evolution of the Dutch High-Prevalent Pseudomonas aeruginosa Clone ST406 during Chronic Colonization of a Patient with Cystic Fibrosis ', P L o S One, vol. 11, no. 6, e0158106 . https://doi.org/10.1371/journal.pone.0158106
- Publication Year :
- 2016
-
Abstract
- This study investigates adaptation of ST406, a prevalent P. aeruginosa clone, present in 15% of chronically infected cystic fibrosis (CF) patients in the Netherlands, in a newly infected CF patient during three years using whole genome sequencing (WGS), transcriptomics, and phenotypic assays, including biofilm formation. WGS-based phylogeny demonstrates that ST406 is genetically distinct from other reported CF related strains or epidemic clones. Comparative genomic analysis of the early (S1) and late (S2) isolate yielded 42 single nucleotide polymorphisms (SNPs) and 10 indels and a single 7 kb genomic fragment only found in S2. Most SNPs and differentially expressed genes encoded proteins involved in metabolism, secretion and signal transduction or transcription. SNPs were identified in regulator genes mexT and exsA and coincided with differential gene expression of mexE and mexF, encoding the MexE/F efflux pump, genes encoding the type six secretion system (T6SS) and type three secretion system (T3SS), which have also been previously implicated in adaptation of other P. aeruginosa strains during chronic infection of CF lungs. The observation that genetically different strains from different patients have accumulated similar genetic adaptations supports the concept of adaptive parallel evolution of P. aeruginosa in chronically infected CF patients. Phenotypically, there was loss of biofilm maturation coinciding with a significant lower level of transcription of both bfmR and bfmS during chronic colonization. These data suggest that the high-prevalent Dutch CF clone ST406 displays adaptation to the CF lung niche, which involves a limited number of mutations affecting regulators controlling biofilm formation and secretion and genes involved in metabolism. These genes could provide good targets for anti-pseudomonal therapy.
- Subjects :
- 0301 basic medicine
Cystic Fibrosis
lcsh:Medicine
medicine.disease_cause
Pathology and Laboratory Medicine
Biochemistry
Genome
Type three secretion system
Medicine and Health Sciences
Prevalence
Ethnicities
lcsh:Science
Phylogeny
Netherlands
Genetics
Medicine(all)
Multidisciplinary
Agricultural and Biological Sciences(all)
Pseudomonas Aeruginosa
High-Throughput Nucleotide Sequencing
Quorum Sensing
Genomics
Phenotype
Bacterial Pathogens
Anti-Bacterial Agents
Medical Microbiology
Pathogens
Sequence Analysis
Transcriptome Analysis
Research Article
Evolutionary Processes
030106 microbiology
Single-nucleotide polymorphism
Microbial Sensitivity Tests
Biology
Opportunistic Infections
Research and Analysis Methods
Microbiology
Polymorphism, Single Nucleotide
03 medical and health sciences
Pseudomonas
Evolutionary Adaptation
medicine
Journal Article
Humans
Pseudomonas Infections
Molecular Biology Techniques
Sequencing Techniques
Gene
Microbial Pathogens
Molecular Biology
Dutch People
Whole genome sequencing
Evolutionary Biology
Bacteria
Pseudomonas aeruginosa
Biochemistry, Genetics and Molecular Biology(all)
Gene Expression Profiling
lcsh:R
Organisms
Sputum
Biology and Life Sciences
Computational Biology
Bacteriology
Molecular Sequence Annotation
Gene Expression Regulation, Bacterial
Genome Analysis
Gene expression profiling
030104 developmental biology
Biofilms
People and Places
lcsh:Q
Population Groupings
Bacterial Biofilms
Transcriptome
Sequence Alignment
Genome, Bacterial
Genetics and Molecular Biology(all)
Cloning
Subjects
Details
- Language :
- English
- ISSN :
- 19326203
- Database :
- OpenAIRE
- Journal :
- PLoS ONE [E], 11(6). Public Library of Science, PLoS ONE, PLoS ONE, Vol 11, Iss 6, p e0158106 (2016), Van Mansfeld, R, De Been, M, Paganelli, F, Yang, L, Bonten, M & Willems, R 2016, ' Within-Host Evolution of the Dutch High-Prevalent Pseudomonas aeruginosa Clone ST406 during Chronic Colonization of a Patient with Cystic Fibrosis ', P L o S One, vol. 11, no. 6, e0158106 . https://doi.org/10.1371/journal.pone.0158106
- Accession number :
- edsair.doi.dedup.....f7b6e401a660031edfb81bf6ad8793da