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Effect of the Surface Charge of Artificial Chaperones on the Refolding of Thermally Denatured Lysozymes
- Source :
- ACS applied materialsinterfaces. 8(6)
- Publication Year :
- 2015
-
Abstract
- Artificial chaperones are of great interest in fighting protein misfolding and aggregation for the protection of protein bioactivity. A comprehensive understanding of the interaction between artificial chaperones and proteins is critical for the effective utilization of these materials in biomedicine. In this work, we fabricated three kinds of artificial chaperones with different surface charges based on mixed-shell polymeric micelles (MSPMs), and investigated their protective effect for lysozymes under thermal stress. It was found that MSPMs with different surface charges showed distinct chaperone-like behavior, and the neutral MSPM with PEG shell and PMEO2MA hydrophobic domain at high temperature is superior to the negatively and positively charged one, because of the excessive electrostatic interactions between the protein and charged MSPMs. The results may benefit to optimize this kind of artificial chaperone with enhanced properties and expand their application in the future.
- Subjects :
- Protein Denaturation
Hot Temperature
02 engineering and technology
010402 general chemistry
01 natural sciences
Micelle
Protein Refolding
Polyethylene Glycols
Avian Proteins
PEG ratio
Animals
Humans
General Materials Science
Surface charge
Micelles
Polymeric micelles
biology
Chemistry
021001 nanoscience & nanotechnology
Electrostatics
0104 chemical sciences
Crystallography
Chaperone (protein)
biology.protein
Biophysics
Protein folding
Muramidase
Self-assembly
0210 nano-technology
Chickens
Molecular Chaperones
Subjects
Details
- ISSN :
- 19448252
- Volume :
- 8
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- ACS applied materialsinterfaces
- Accession number :
- edsair.doi.dedup.....f7c7855da885b06b42fc7032f257359a