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miR-196B-5P and miR-200B-3P Are Differentially Expressed in Medulloblastomas of Adults and Children
- Source :
- Diagnostics, Diagnostics, Vol 10, Iss 265, p 265 (2020), Volume 10, Issue 5
- Publication Year :
- 2020
-
Abstract
- Medulloblastoma is a highly aggressive brain tumor that typically affects children, while in adults it represents ~1% of all brain tumors. Little is known about microRNA expression profile of the rare adult medulloblastoma. The main aim of this study was to identify peculiar differences in microRNA expression between childhood and adult medulloblastoma. Medulloblastomas were profiled for microRNA expression using the Exiqon Human miRNome panel (I + II) analyzing 752 microRNAs in a training set of six adult and six childhood cases. Then, the most differentially expressed microRNAs were validated in a total of 21 adult and 19 childhood cases. Eight microRNAs (miR-196b-5p, miR-183-5p, miR-200b-3p, miR-196a-5p, miR-193a-3p, miR-29c-3p, miR-33b-5p, and miR-200a-3p) were differentially expressed in medulloblastoma of adults and children. Analysis of the validation set confirmed that miR-196b-5p and miR-200b-3p were significantly overexpressed in medulloblastoma of adults as compared with those of children. We followed an in silico approach to investigate direct targets and the pathways involved for the two microRNAs (miR-196b and miR-200b) differently expressed between adult and childhood medulloblastoma. Adult and childhood medulloblastoma have different miRNA expression profiles. In particular, the differential dysregulation of miR-196b-5p and miR-200b-3p characterizes the miRNA profile of adult medulloblastoma and suggests potential targets for novel diagnostic, prognostic, or therapeutic strategies.
- Subjects :
- 0301 basic medicine
Oncology
medicine.medical_specialty
Adult Medulloblastoma
In silico
Clinical Biochemistry
Brain tumor
Biology
Article
03 medical and health sciences
0302 clinical medicine
Internal medicine
microRNA
medicine
Childhood Medulloblastoma
microRNA profile
neoplasms
Medulloblastoma
lcsh:R5-920
adult medulloblastoma
Correction
MicroRNA Expression Profile
medicine.disease
childhood medulloblastoma
nervous system diseases
stomatognathic diseases
030104 developmental biology
030220 oncology & carcinogenesis
Mir 200c
lcsh:Medicine (General)
Subjects
Details
- ISSN :
- 20754418
- Volume :
- 10
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Diagnostics (Basel, Switzerland)
- Accession number :
- edsair.doi.dedup.....f7e9d35352351784a21c65b3ab0dc40a